EDTA enhances cementum-like tissue formation, TGF-β1 & VEGF in rat molars during regenerative endodontics
Abstract This study investigated the influence of ethylenediaminetetraacetic acid (EDTA) irrigation on cementum-like tissue formation and TGF-β1, FGF-2, and VEGF immunolabeling during regenerative endodontic procedures (REPs) in immature rat molars. The lower first molars of 12 four-week-old male ra...
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Sociedade Brasileira de Pesquisa Odontológica
2025-06-01
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| Series: | Brazilian Oral Research |
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| author | Alexandre Henrique dos REIS-PRADO Isabela Joane Prado SILVA Juliana GOTO Nathália Evelyn da Silva MACHADO Gleide Fernandes de AVELAR Juliano Douglas Silva ALBERGARIA Raphael Escorsim SZAWKA Marco Cícero BOTTINO Luciano Tavares Angelo CINTRA Edilson ERVOLINO Antônio Paulino RIBEIRO-SOBRINHO Francine BENETTI |
| author_facet | Alexandre Henrique dos REIS-PRADO Isabela Joane Prado SILVA Juliana GOTO Nathália Evelyn da Silva MACHADO Gleide Fernandes de AVELAR Juliano Douglas Silva ALBERGARIA Raphael Escorsim SZAWKA Marco Cícero BOTTINO Luciano Tavares Angelo CINTRA Edilson ERVOLINO Antônio Paulino RIBEIRO-SOBRINHO Francine BENETTI |
| author_sort | Alexandre Henrique dos REIS-PRADO |
| collection | DOAJ |
| description | Abstract This study investigated the influence of ethylenediaminetetraacetic acid (EDTA) irrigation on cementum-like tissue formation and TGF-β1, FGF-2, and VEGF immunolabeling during regenerative endodontic procedures (REPs) in immature rat molars. The lower first molars of 12 four-week-old male rats (80 g) underwent pulpectomy in the mesial canal and were randomly categorized into two experimental groups (n = 6): sodium hypochlorite (NaOCl) – irrigated for 5 min with 2.5% NaOCl; and NaOCl-EDTA – irrigated with 2.5% NaOCl, followed by 5 min of 17% EDTA. After inducing bleeding with a size 10 K-file, the cavities were sealed. Untreated molars served as control-15d (n = 3) and immediate control (n = 3). Either immediately or after 15 days, the animals were euthanized, and the teeth were collected for histomorphometric and immunohistochemical (TGF-β1, FGF-2, and VEGF) analysis. The results were analyzed by the Mann-Whitney U-test (p < 0.05). Histomorphometric analysis revealed increased cementum-like tissue formation in the NaOCl-EDTA group compared with that in the NaOCl group (p < 0.05). Regarding growth factor immunolabeling, the NaOCl-EDTA group exhibited enhanced TGF-β1 and VEGF immunolabeling in the root tip area and the center region of the apical third of the pulp tissue, compared with that in the NaOCl group (p < 0.05); however, no significant difference was observed in FGF-2 (p > 0.05). In conclusion, the use of EDTA in REPs positively affected the formation of cementum-like tissue and TGF-β1 and VEGF in the apical region but did not influence FGF-2. |
| format | Article |
| id | doaj-art-db54d9d9023845bcb366e3d5dff8eb9b |
| institution | Matheson Library |
| issn | 1807-3107 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
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| series | Brazilian Oral Research |
| spelling | doaj-art-db54d9d9023845bcb366e3d5dff8eb9b2025-07-01T07:43:37ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1807-31072025-06-013910.1590/1807-3107bor-2025.vol39.068EDTA enhances cementum-like tissue formation, TGF-β1 & VEGF in rat molars during regenerative endodonticsAlexandre Henrique dos REIS-PRADOhttps://orcid.org/0000-0002-5866-7137Isabela Joane Prado SILVAhttps://orcid.org/0000-0002-0103-8946Juliana GOTOhttps://orcid.org/0000-0002-6040-3560Nathália Evelyn da Silva MACHADOhttps://orcid.org/0000-0002-4196-782XGleide Fernandes de AVELARhttps://orcid.org/0000-0002-2132-3218Juliano Douglas Silva ALBERGARIARaphael Escorsim SZAWKAhttps://orcid.org/0000-0002-2639-3469Marco Cícero BOTTINOhttps://orcid.org/0000-0001-8740-2464Luciano Tavares Angelo CINTRAhttps://orcid.org/0000-0003-2348-7846Edilson ERVOLINOhttps://orcid.org/0000-0003-4859-0583Antônio Paulino RIBEIRO-SOBRINHOhttps://orcid.org/0000-0002-3598-7592Francine BENETTIhttps://orcid.org/0000-0002-5459-353XAbstract This study investigated the influence of ethylenediaminetetraacetic acid (EDTA) irrigation on cementum-like tissue formation and TGF-β1, FGF-2, and VEGF immunolabeling during regenerative endodontic procedures (REPs) in immature rat molars. The lower first molars of 12 four-week-old male rats (80 g) underwent pulpectomy in the mesial canal and were randomly categorized into two experimental groups (n = 6): sodium hypochlorite (NaOCl) – irrigated for 5 min with 2.5% NaOCl; and NaOCl-EDTA – irrigated with 2.5% NaOCl, followed by 5 min of 17% EDTA. After inducing bleeding with a size 10 K-file, the cavities were sealed. Untreated molars served as control-15d (n = 3) and immediate control (n = 3). Either immediately or after 15 days, the animals were euthanized, and the teeth were collected for histomorphometric and immunohistochemical (TGF-β1, FGF-2, and VEGF) analysis. The results were analyzed by the Mann-Whitney U-test (p < 0.05). Histomorphometric analysis revealed increased cementum-like tissue formation in the NaOCl-EDTA group compared with that in the NaOCl group (p < 0.05). Regarding growth factor immunolabeling, the NaOCl-EDTA group exhibited enhanced TGF-β1 and VEGF immunolabeling in the root tip area and the center region of the apical third of the pulp tissue, compared with that in the NaOCl group (p < 0.05); however, no significant difference was observed in FGF-2 (p > 0.05). In conclusion, the use of EDTA in REPs positively affected the formation of cementum-like tissue and TGF-β1 and VEGF in the apical region but did not influence FGF-2.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242025000100556&lng=en&tlng=enEdetic AcidFibroblast Growth Factor 2Regenerative EndodonticsTransforming Growth Factor betaVascular Endothelial Growth Factor A |
| spellingShingle | Alexandre Henrique dos REIS-PRADO Isabela Joane Prado SILVA Juliana GOTO Nathália Evelyn da Silva MACHADO Gleide Fernandes de AVELAR Juliano Douglas Silva ALBERGARIA Raphael Escorsim SZAWKA Marco Cícero BOTTINO Luciano Tavares Angelo CINTRA Edilson ERVOLINO Antônio Paulino RIBEIRO-SOBRINHO Francine BENETTI EDTA enhances cementum-like tissue formation, TGF-β1 & VEGF in rat molars during regenerative endodontics Brazilian Oral Research Edetic Acid Fibroblast Growth Factor 2 Regenerative Endodontics Transforming Growth Factor beta Vascular Endothelial Growth Factor A |
| title | EDTA enhances cementum-like tissue formation, TGF-β1 & VEGF in rat molars during regenerative endodontics |
| title_full | EDTA enhances cementum-like tissue formation, TGF-β1 & VEGF in rat molars during regenerative endodontics |
| title_fullStr | EDTA enhances cementum-like tissue formation, TGF-β1 & VEGF in rat molars during regenerative endodontics |
| title_full_unstemmed | EDTA enhances cementum-like tissue formation, TGF-β1 & VEGF in rat molars during regenerative endodontics |
| title_short | EDTA enhances cementum-like tissue formation, TGF-β1 & VEGF in rat molars during regenerative endodontics |
| title_sort | edta enhances cementum like tissue formation tgf β1 vegf in rat molars during regenerative endodontics |
| topic | Edetic Acid Fibroblast Growth Factor 2 Regenerative Endodontics Transforming Growth Factor beta Vascular Endothelial Growth Factor A |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242025000100556&lng=en&tlng=en |
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