Optional medium, initial inoculum and pH control for mass production of the entomopathogenic fungus Paecilomyces fumosoroseus in submerged culture
The entomopathogenic fungal isolate Paecilomyces fumosoroseus Pfr116 highly infective to sucking insects was cultured in submerged conditions at 25℃ using five liquid media. Based on the yield of dry mycelia over incubation time, a low-cost medium was selected for use in mass production from the fiv...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2003-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2003.01.0039 |
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| Summary: | The entomopathogenic fungal isolate Paecilomyces fumosoroseus Pfr116 highly infective to sucking insects was cultured in submerged conditions at 25℃ using five liquid media. Based on the yield of dry mycelia over incubation time, a low-cost medium was selected for use in mass production from the five media tested. Its components (w/v) included 2% sucrose, 20% potato, and 0.1% peptone supplemented with 0.01% NaNO<sub>3</sub>, 0.01% KCl, 0.025% MgSO<sub>4</sub>, 0.005% MnSO<sub>4</sub>, 0.005% FeSO<sub>4</sub> and 0.05% KH<sub>2</sub>PO<sub>4</sub>. A 48-h culture of the medium resulted in a mycelial yield of>20 mg/mL, which was close to the mycelial yield of Sabouraud dextrose broth during the same period of incubation. Other optional conditions for initiating the culture included: ① the amount of initial inoculum to guarantee the liquid containing mycelial biomass of 1.5-2.0 mg/mL; and ② a pH ranging from 5 to 7. |
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| ISSN: | 1008-9209 2097-5155 |