Osteoclast visualization: Tartrate-resistant acid phosphatase activity staining using NewFuchsin compatible with non-aqueous mounting and tissue clearing

Tartrate-resistant acid phosphatase (TRAP) staining is widely used to stain osteoclasts in histological bone sections. The red dye formed by the conventional TRAP enzymatic reaction using naphthol AS-MX (or AS-BI) phosphate and fast red-violet (or garnet) chromogens is readily soluble in alcohol or...

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Bibliographic Details
Main Authors: Takashi Nakamura, Katsuhiro Kawaai, Yukiko Kuroda, Koichi Matsuo
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:MethodsX
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Online Access:http://www.sciencedirect.com/science/article/pii/S2215016124005879
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