Construction of Paecilomyces fumosoroseus protoplast-transforming system by restriction enzyme-mediated integration (REMI)

Phosphinothricin resistance gene Bar vectored by prepared plasmid pAN52-1N-Bar was transformed into the protoplasts of the entomopathogenic fungus Paecilomyces fumosoroseus Pfr116 using the method of restriction enzyme-mediated integration (REMI). The protoplast-based transformation resulted in 6-13...

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Bibliographic Details
Main Authors: YANG Xu-fen, YING Sheng-hua, FENG Ming-guang
Format: Article
Language:English
Published: Zhejiang University Press 2006-11-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/1008-9209.2006.06.0606
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