Acaricidal Activity of Biosurfactants Produced by <i>Serratia ureilytica</i> on <i>Tetranychus urticae</i> and Their Compatibility with the Predatory Mite <i>Amblyseius swirskii</i>
This study evaluated the acaricidal effects of biosurfactants produced by <i>Serratia ureilytica</i> against the two-spotted spider mite <i>Tetranychus urticae</i> and their compatibility with the predatory mite <i>Ambliseus swirski</i>. The biosurfactants were ob...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
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| Series: | Microbiology Research |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2036-7481/16/7/150 |
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| Summary: | This study evaluated the acaricidal effects of biosurfactants produced by <i>Serratia ureilytica</i> against the two-spotted spider mite <i>Tetranychus urticae</i> and their compatibility with the predatory mite <i>Ambliseus swirski</i>. The biosurfactants were obtained via liquid cultures of the bacterial strains. In the laboratory, <i>T. urticae</i> was exposed via acaricide-immersed leaves and <i>A. swirskii</i> via acaricide-coated glass vials. In the greenhouse, mite-infested plants were sprayed with the biosurfactants. In the laboratory, biosurfactants produced by <i>S. ureilytica</i> NOD-3 and UTS exhibited strong acaricidal activity, causing 95% mortality in adults and reducing egg viability by more than 60%. In the greenhouse trial, all biosurfactants significantly suppressed <i>T. urticae</i> populations at all evaluated periods (7, 14, and 21 days post-application). Gas chromatography–mass spectrometry (GC-MS) analysis of the biosurfactants identified several fatty acids, including hexadecanoic acid, pentanoic acid, octadecanoic acid, decanoic acid, and tetradecanoic acid, as well as the amino acids L-proline, L-lysine, L-valine, and glutamic acid. These fatty acids and amino acids are known structural components of lipopeptides. Furthermore, the bioinformatic analysis of the genomes of the three <i>S. ureilytica</i> strains revealed nonribosomal peptide synthetase (NRPS) gene clusters homologous to those involved in the biosynthesis of lipopeptides. These findings demonstrate that <i>S. ureilytica</i> biosurfactants are promising eco-friendly acaricides, reducing <i>T. urticae</i> populations by >95% while partially sparing <i>A. swirskii</i>. |
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| ISSN: | 2036-7481 |