Cloning and Expression of β-1, 3-1, 4-glucanase Gene from Bacillus pumilus

A gene encoding β-glucanase from Bacillus pumilus has been cloned and expressed in Escherichia coli. The whole length of the gene was 754 bp, including an ORF of 717 bp encoding 239 amino acids. The enzyme showed a molecular size of 26.98 kD and a pI of 6.08. The gene showed a high degree of similar...

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Bibliographic Details
Main Authors: LÜ Wen-ping, XU Zi-rong, SUN Jian-yi, LI Wei-fen, DU Wen-li
Format: Article
Language:English
Published: Zhejiang University Press 2004-11-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/1008-9209.2004.06.0679
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