Laboratory Test Indirectly Reveals the Unreliability of RNA-Dependent 16S rRNA Amplicon Sequences in Detecting the Gut Bacterial Diversity of <i>Delia antiqua</i>

Laboratory Test Indirectly Reveals the Unreliability of RNA-Dependent 16S rRNA Amplicon Sequences in Detecting the Gut Bacterial Diversity of <i>Delia antiqua</i>

In insect–microbe symbiosis, understanding the diversity of associated bacteria is crucial. DNA-dependent sequence methods are widely used to assess microbial diversity in insects, but they cannot distinguish between live and dead microbes. In contrast, RNA-dependent sequencing can identify alive ba...

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Bibliographic Details
Main Authors: Miaomiao Li, Xin Cao, Linfeng Xu, Luyao Lin, Xiaoqing Wu, Susu Fan, Xinjian Zhang, Fangyuan Zhou
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Insects
Subjects:
gut microbe
symbiont
onion maggot
amplicon sequence
insect–microbe
symbiosis
Online Access:https://www.mdpi.com/2075-4450/16/6/611
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