Phenotypic Profiling of Biofilm Development and Efflux Pump Mechanisms in Multidrug-Resistant (MDR) Klebsiella pneumoniae
Study’s Excerpt: • All pneumoniae isolates were multidrug-resistant. • High resistance seen to Ofloxacin (75%) and Cephalexin (56%). • 25% of isolates formed biofilms; 12.5% showed efflux pump activity. • Ethidium bromide and Congo red agar were used for phenotypic tests. • Biofilms an...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Umaru Musa Yar'adua University, Katsina, Nigeria
2025-06-01
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| Series: | UMYU Journal of Microbiology Research |
| Subjects: | |
| Online Access: | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/1150 |
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| Summary: | Study’s Excerpt:
• All pneumoniae isolates were multidrug-resistant.
• High resistance seen to Ofloxacin (75%) and Cephalexin (56%).
• 25% of isolates formed biofilms; 12.5% showed efflux pump activity.
• Ethidium bromide and Congo red agar were used for phenotypic tests.
• Biofilms and efflux pumps contribute to antibiotic resistance.
Full Abstract:
Klebsiella pneumoniae causes diverse healthcare- and community-associated infections, frequently affecting patients with indwelling medical devices. Its ability to form biofilms and activate efflux pumps enhances antibiotic resistance. These two mechanisms work synergistically, accelerating the emergence of multidrug-resistant K. pneumoniae strains..This study investigated the antibiotic resistance patterns of Klebsiella pneumoniae isolates in relation to their potential for biofilm formation and efflux pump activity. A total of Sixteen (16) K. pneumoniae isolates were obtained from the Department of Microbiology, University of Lagos, Lagos State. Confirmation of K. pneumoniae was conducted through culture, microscopy, and biochemical assays. Antibiotic susceptibility testing was performed using six routinely prescribed antibiotics. Phenotypic assessment of efflux pump activity and biofilm formation was conducted using the ethidium bromide (EtBr) cartwheel assay and Congo red agar method, respectively. The results indicated that all isolates were multidrug-resistant, exhibiting high resistance to Ofloxacin (12, 75.00%), Cephalexin (9, 56.25%), and Ampicillin (8, 50.00%). Four 4(25%)isolates demonstrated biofilm-forming ability, while only two 2(12.5%) isolates exhibited efflux pump activity. The results highlight biofilm formation and efflux pump activity as key virulence mechanisms contributing to the pathogenicity and multidrug resistance of K. pneumoniae, highlighting the need for targeted therapeutic strategies to overcome these resistance mechanisms.
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| ISSN: | 2616-0668 2814-1822 |