Overproduction of Tk-Subtilisin and Elucidation of Its Structural Thermostability Based on Molecular Dynamics Studies

Abstract Background: Subtilisin from Thermococcus kodakaraensis (Tk-subtilisin) is an ideal candidate for many industrial and research processes due to its unique features. Methods: In this study, the enzyme is overproduced in E. coli using four different media i.e. YNG, M9NG, TB and LB and its th...

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Bibliographic Details
Main Authors: Nouman Rasool, Naeem Rashid
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2025-07-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132025000100406&lng=en&tlng=en
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