In Vitro Evaluation of the Ovicidal Potential of Proteases from <i>Beauveria bassiana</i> Against <i>Eurytrema pancreaticum</i> Eggs

In Vitro Evaluation of the Ovicidal Potential of Proteases from <i>Beauveria bassiana</i> Against <i>Eurytrema pancreaticum</i> Eggs

In the search for new alternatives for controlling parasitic agents, proteases from <i>Beauveria bassiana</i> stand out. The aim of this study was to evaluate in silico and in vitro the ovicidal potential of <i>B. bassiana</i> proteases on <i>Eurytrema pancreaticum</...

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Main Authors: Lisseth Bibiana Puentes Figueroa, Amanda do Carmo Alves, Adriane Toledo da Silva, Debora Castro de Souza, Nivia Kelly Lima Sales, Lorrana Verdi Flores, Tiago Facury Moreira, Fabio Ribeiro Braga, Filippe Elias de Freitas Soares
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Microbiology Research
Subjects:
entomopathogenic fungus
protease
eurythrematosis
3D structure
zymogram
Online Access:https://www.mdpi.com/2036-7481/16/6/127
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Summary:In the search for new alternatives for controlling parasitic agents, proteases from <i>Beauveria bassiana</i> stand out. The aim of this study was to evaluate in silico and in vitro the ovicidal potential of <i>B. bassiana</i> proteases on <i>Eurytrema pancreaticum</i> Janson, 1889 (Dicrocoeliidae) eggs. <i>Beauveria bassiana</i> Bals. -Criv., 1835 (Cordycipitaceae) (IP 361) was cultivated for enzymatic production. Proteins were precipitated with acetone (1:4 ratio), and specific activity was determined. Protease profiles were assessed via zymography, and inhibition by phenylmethylsulfonyl fluoride (PMSF) and ethylenediaminetetraacetic acid (EDTA) was tested. Three-dimensional models of the proteases were generated. <i>Eurytrema pancreaticum</i> eggs were used for the in vitro anthelmintic evaluation of the proteases. The results showed that precipitation significantly concentrated proteolytic activity (<i>p</i> < 0.01) compared to the crude extract. However, no chitinase activity was detected. The proteolytic profile of the precipitate revealed five bands with molecular weights from 25.6 to 66.9 kDa. In the in vitro tests, the proteases significantly (<i>p</i> < 0.01) reduced the number of intact <i>E. pancreaticum</i> eggs by 53% compared to the control with denatured enzymes. These findings highlight the ovicidal potential of <i>B. bassiana</i> proteases, though further studies are needed to confirm their application in parasite control.
ISSN:2036-7481

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