EDTA enhances cementum-like tissue formation, TGF-β1 & VEGF in rat molars during regenerative endodontics

EDTA enhances cementum-like tissue formation, TGF-β1 & VEGF in rat molars during regenerative endodontics

Abstract This study investigated the influence of ethylenediaminetetraacetic acid (EDTA) irrigation on cementum-like tissue formation and TGF-β1, FGF-2, and VEGF immunolabeling during regenerative endodontic procedures (REPs) in immature rat molars. The lower first molars of 12 four-week-old male ra...

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Main Authors: Alexandre Henrique dos REIS-PRADO, Isabela Joane Prado SILVA, Juliana GOTO, Nathália Evelyn da Silva MACHADO, Gleide Fernandes de AVELAR, Juliano Douglas Silva ALBERGARIA, Raphael Escorsim SZAWKA, Marco Cícero BOTTINO, Luciano Tavares Angelo CINTRA, Edilson ERVOLINO, Antônio Paulino RIBEIRO-SOBRINHO, Francine BENETTI
Format: Article
Language:English
Published: Sociedade Brasileira de Pesquisa Odontológica 2025-06-01
Series:Brazilian Oral Research
Subjects:
Edetic Acid
Fibroblast Growth Factor 2
Regenerative Endodontics
Transforming Growth Factor beta
Vascular Endothelial Growth Factor A
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242025000100556&lng=en&tlng=en
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Summary:Abstract This study investigated the influence of ethylenediaminetetraacetic acid (EDTA) irrigation on cementum-like tissue formation and TGF-β1, FGF-2, and VEGF immunolabeling during regenerative endodontic procedures (REPs) in immature rat molars. The lower first molars of 12 four-week-old male rats (80 g) underwent pulpectomy in the mesial canal and were randomly categorized into two experimental groups (n = 6): sodium hypochlorite (NaOCl) – irrigated for 5 min with 2.5% NaOCl; and NaOCl-EDTA – irrigated with 2.5% NaOCl, followed by 5 min of 17% EDTA. After inducing bleeding with a size 10 K-file, the cavities were sealed. Untreated molars served as control-15d (n = 3) and immediate control (n = 3). Either immediately or after 15 days, the animals were euthanized, and the teeth were collected for histomorphometric and immunohistochemical (TGF-β1, FGF-2, and VEGF) analysis. The results were analyzed by the Mann-Whitney U-test (p < 0.05). Histomorphometric analysis revealed increased cementum-like tissue formation in the NaOCl-EDTA group compared with that in the NaOCl group (p < 0.05). Regarding growth factor immunolabeling, the NaOCl-EDTA group exhibited enhanced TGF-β1 and VEGF immunolabeling in the root tip area and the center region of the apical third of the pulp tissue, compared with that in the NaOCl group (p < 0.05); however, no significant difference was observed in FGF-2 (p > 0.05). In conclusion, the use of EDTA in REPs positively affected the formation of cementum-like tissue and TGF-β1 and VEGF in the apical region but did not influence FGF-2.
ISSN:1807-3107

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