Optimum conditions of in vitro proliferation of splenic lymphocyte from Chinese soft-shelled turtle (Trionyx sinensis)
In order to optimize the conditions for in vitro proliferation of splenic lymphocyte of Chinese Soft-shelled turtle (Trionyx sinensis), four main parameters were tested including different kinds and concentrations of mitogens, cell density and incubation time. By colorimetric method utilizing MTT [3...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2008-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.04.006 |
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| Summary: | In order to optimize the conditions for in vitro proliferation of splenic lymphocyte of Chinese Soft-shelled turtle (Trionyx sinensis), four main parameters were tested including different kinds and concentrations of mitogens, cell density and incubation time. By colorimetric method utilizing MTT [3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide], the lymphocyte at a density of 2×10<sup>6</sup> cells·mL<sup>-1</sup> incubated with 10 μg·mL<sup>-1</sup> concanavalin A (Con A) for 120 hours or 5 μg·mL<sup>-1</sup> phytohaemagglutinin (PHA) for 144 hours was finally determined as the optimum conditions for proliferation. The results above indicate that MTT might be a reliable method for in vitro proliferation of splenic lymphocyte in Chinese soft-shelled turtle. |
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| ISSN: | 1008-9209 2097-5155 |