Optimum conditions of in vitro proliferation of splenic lymphocyte from Chinese soft-shelled turtle (Trionyx sinensis)

In order to optimize the conditions for in vitro proliferation of splenic lymphocyte of Chinese Soft-shelled turtle (Trionyx sinensis), four main parameters were tested including different kinds and concentrations of mitogens, cell density and incubation time. By colorimetric method utilizing MTT [3...

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Bibliographic Details
Main Authors: LI Xiao-liang, LIU Gang, ZHANG Yan-ming, SHUAI Jiang-bing, FANG Wei-huan
Format: Article
Language:English
Published: Zhejiang University Press 2008-07-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.04.006
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