Analytical Characterization of Medium Molecular Weight Chitosan from Pink Shrimp (Metapenaeus dobsoni) Shells
Seafood is widely consumed across the globe and serves as a vital source of essential nutrients. However, the processing of seafood generates significant amounts of waste, including shells and scales, which hold potential for the production of valuable byproducts. This study presents an efficient me...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
National Centre of Excellence in Analytical Chemsitry
2025-06-01
|
| Series: | Pakistan Journal of Analytical & Environmental Chemistry |
| Subjects: | |
| Online Access: | https://pjaec.pk/index.php/pjaec/article/view/321/262 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839651448559763456 |
|---|---|
| author | Ammara Tooba Akbar Mahnaz Ahmad Rashida Rahmat Zohra Raheela Rahmat Zohra Ahmad Khan Muhammad Atiq Ur Rehman Mohamed Abbas |
| author_facet | Ammara Tooba Akbar Mahnaz Ahmad Rashida Rahmat Zohra Raheela Rahmat Zohra Ahmad Khan Muhammad Atiq Ur Rehman Mohamed Abbas |
| author_sort | Ammara Tooba Akbar |
| collection | DOAJ |
| description | Seafood is widely consumed across the globe and serves as a vital source of essential nutrients. However, the processing of seafood generates significant amounts of waste, including shells and scales, which hold potential for the production of valuable byproducts. This study presents an efficient method for synthesizing high-quality chitosan from Pink shrimp (Metapenaeus dobsoni) shells. The extraction process involves a novel two-step purification of chitin, followed by an energy-saving freeze-pump-out-thaw (FPT) cycle and optimized deacetylation to obtain chitosan. Physicochemical characterization revealed that the chitosan has an average molecular weight (Mv) of 620 kDa and is soluble in 1% acetic acid. Scanning electron microscopy (SEM) confirmed the conformation of the synthesized chitosan. The degree of deacetylation was determined to be 97.2% using Fourier-transform infrared spectroscopy (FTIR), while the crystallinity index (Icr) was calculated at 69% via powdered X-ray diffraction (XRD). The findings demonstrated that the chitosan extracted from this crustacean source possesses unique physical and chemical properties, making it highly suitable for applications in biomedical fields and food packaging. |
| format | Article |
| id | doaj-art-ba9ba5d376294d78be788c897cd8df2c |
| institution | Matheson Library |
| issn | 1996-918X 2221-5255 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | National Centre of Excellence in Analytical Chemsitry |
| record_format | Article |
| series | Pakistan Journal of Analytical & Environmental Chemistry |
| spelling | doaj-art-ba9ba5d376294d78be788c897cd8df2c2025-06-26T06:08:06ZengNational Centre of Excellence in Analytical ChemsitryPakistan Journal of Analytical & Environmental Chemistry1996-918X2221-52552025-06-012601394810.21743/pjaec/2025.06.04Analytical Characterization of Medium Molecular Weight Chitosan from Pink Shrimp (Metapenaeus dobsoni) ShellsAmmara Tooba Akbar0Mahnaz Ahmad1Rashida Rahmat Zohra2Raheela Rahmat Zohra3Ahmad Khan4Muhammad Atiq Ur Rehman5Mohamed Abbas6Department of Biotechnology, University of Karachi, Karachi-75270, Pakistan.Department of Biotechnology, University of Karachi, Karachi-75270, Pakistan.Department of Biotechnology, Jinnah University for Women, Karachi-74600, Pakistan.Department of Biotechnology, University of Karachi, Karachi-75270, Pakistan.Department of Materials Science and Engineering, Institute of Space Technology, Islamabad-44000, Pakistan.Department of Materials Science and Engineering, Institute of Space Technology, Islamabad-44000, Pakistan.Electrical Engineering Department, College of Engineering, King Khalid University, Abha 61421, Saudi Arabia.Seafood is widely consumed across the globe and serves as a vital source of essential nutrients. However, the processing of seafood generates significant amounts of waste, including shells and scales, which hold potential for the production of valuable byproducts. This study presents an efficient method for synthesizing high-quality chitosan from Pink shrimp (Metapenaeus dobsoni) shells. The extraction process involves a novel two-step purification of chitin, followed by an energy-saving freeze-pump-out-thaw (FPT) cycle and optimized deacetylation to obtain chitosan. Physicochemical characterization revealed that the chitosan has an average molecular weight (Mv) of 620 kDa and is soluble in 1% acetic acid. Scanning electron microscopy (SEM) confirmed the conformation of the synthesized chitosan. The degree of deacetylation was determined to be 97.2% using Fourier-transform infrared spectroscopy (FTIR), while the crystallinity index (Icr) was calculated at 69% via powdered X-ray diffraction (XRD). The findings demonstrated that the chitosan extracted from this crustacean source possesses unique physical and chemical properties, making it highly suitable for applications in biomedical fields and food packaging.https://pjaec.pk/index.php/pjaec/article/view/321/262chitinchitosanbiopolymershrimp shells wastepurification |
| spellingShingle | Ammara Tooba Akbar Mahnaz Ahmad Rashida Rahmat Zohra Raheela Rahmat Zohra Ahmad Khan Muhammad Atiq Ur Rehman Mohamed Abbas Analytical Characterization of Medium Molecular Weight Chitosan from Pink Shrimp (Metapenaeus dobsoni) Shells Pakistan Journal of Analytical & Environmental Chemistry chitin chitosan biopolymer shrimp shells waste purification |
| title | Analytical Characterization of Medium Molecular Weight Chitosan from Pink Shrimp (Metapenaeus dobsoni) Shells |
| title_full | Analytical Characterization of Medium Molecular Weight Chitosan from Pink Shrimp (Metapenaeus dobsoni) Shells |
| title_fullStr | Analytical Characterization of Medium Molecular Weight Chitosan from Pink Shrimp (Metapenaeus dobsoni) Shells |
| title_full_unstemmed | Analytical Characterization of Medium Molecular Weight Chitosan from Pink Shrimp (Metapenaeus dobsoni) Shells |
| title_short | Analytical Characterization of Medium Molecular Weight Chitosan from Pink Shrimp (Metapenaeus dobsoni) Shells |
| title_sort | analytical characterization of medium molecular weight chitosan from pink shrimp metapenaeus dobsoni shells |
| topic | chitin chitosan biopolymer shrimp shells waste purification |
| url | https://pjaec.pk/index.php/pjaec/article/view/321/262 |
| work_keys_str_mv | AT ammaratoobaakbar analyticalcharacterizationofmediummolecularweightchitosanfrompinkshrimpmetapenaeusdobsonishells AT mahnazahmad analyticalcharacterizationofmediummolecularweightchitosanfrompinkshrimpmetapenaeusdobsonishells AT rashidarahmatzohra analyticalcharacterizationofmediummolecularweightchitosanfrompinkshrimpmetapenaeusdobsonishells AT raheelarahmatzohra analyticalcharacterizationofmediummolecularweightchitosanfrompinkshrimpmetapenaeusdobsonishells AT ahmadkhan analyticalcharacterizationofmediummolecularweightchitosanfrompinkshrimpmetapenaeusdobsonishells AT muhammadatiqurrehman analyticalcharacterizationofmediummolecularweightchitosanfrompinkshrimpmetapenaeusdobsonishells AT mohamedabbas analyticalcharacterizationofmediummolecularweightchitosanfrompinkshrimpmetapenaeusdobsonishells |