Development of a DNA Aptamer‐Based Approach to Noninvasively Image CAR‐T Cells In Vivo and Traceless Enrichment In Vitro
Abstract Chimeric antigen receptor (CAR) T cells offered a potential cure for malignancies, however, their outcomes and dynamics across different anatomical sites remained inadequately characterized. Monitoring the bio‐distribution and tumor‐homing of CAR‐T cells in vivo is crucial, as it provides p...
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| Format: | Article |
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Wiley
2025-07-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202506746 |
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| author | Minghui Chen Pengzhao Chang Zhen Zhang Dan Liu Rui Hou Ming Shi Jingjing Li Kai Xu Junnian Zheng |
| author_facet | Minghui Chen Pengzhao Chang Zhen Zhang Dan Liu Rui Hou Ming Shi Jingjing Li Kai Xu Junnian Zheng |
| author_sort | Minghui Chen |
| collection | DOAJ |
| description | Abstract Chimeric antigen receptor (CAR) T cells offered a potential cure for malignancies, however, their outcomes and dynamics across different anatomical sites remained inadequately characterized. Monitoring the bio‐distribution and tumor‐homing of CAR‐T cells in vivo is crucial, as it provides patient‐specific data that might inform on treatment success, potential failure, and off‐target toxicities. Herein, an Aptamer A3 by Cell‐SELEX (systematic evolution of ligands by exponential enrichment) is generated, which can bind with CAR‐T cells with nanomolar affinity. After CAR‐T cells are injected into Nalm6 xenograft tumor model mice through tail vein, Cy5‐labeled A3 is injected into mice for fluorescence time‐delay imaging in vivo. The fluorescence signal produced by the Cy5‐labeled A3 is accumulated in the tumor area and reached its maximum at day 14. Moreover, A3 could enrich CAR‐T cells in mixed cell populations in a traceless way. A3 is screened for CAR‐T cells imaging and CAR‐T cells enrichment, which may provide assistance for the evaluation of CAR‐T cells efficacy and the manufacture of CAR‐T cells. Overall, this research shows that A3 enabled repeated, sensitive, and specific assessment of the infused CAR‐T cells in vivo. The screened aptamer will have broad applications for tracking CAR‐T cells in patients, providing insights into treatment success, potential failure, and off‐target toxicities. |
| format | Article |
| id | doaj-art-a42e2f1f00ad4bdf8c20b1572da3de62 |
| institution | Matheson Library |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-a42e2f1f00ad4bdf8c20b1572da3de622025-07-24T12:06:19ZengWileyAdvanced Science2198-38442025-07-011228n/an/a10.1002/advs.202506746Development of a DNA Aptamer‐Based Approach to Noninvasively Image CAR‐T Cells In Vivo and Traceless Enrichment In VitroMinghui Chen0Pengzhao Chang1Zhen Zhang2Dan Liu3Rui Hou4Ming Shi5Jingjing Li6Kai Xu7Junnian Zheng8School of Medical Imaging Xuzhou Medical University Xuzhou Jiangsu 221004 ChinaSchool of Medical Imaging Xuzhou Medical University Xuzhou Jiangsu 221004 ChinaSchool of Medical Imaging Xuzhou Medical University Xuzhou Jiangsu 221004 ChinaCancer Institute Xuzhou Medical University Xuzhou 221002 ChinaCancer Institute Xuzhou Medical University Xuzhou 221002 ChinaCancer Institute Xuzhou Medical University Xuzhou 221002 ChinaSchool of Medical Imaging Xuzhou Medical University Xuzhou Jiangsu 221004 ChinaSchool of Medical Imaging Xuzhou Medical University Xuzhou Jiangsu 221004 ChinaCancer Institute Xuzhou Medical University Xuzhou 221002 ChinaAbstract Chimeric antigen receptor (CAR) T cells offered a potential cure for malignancies, however, their outcomes and dynamics across different anatomical sites remained inadequately characterized. Monitoring the bio‐distribution and tumor‐homing of CAR‐T cells in vivo is crucial, as it provides patient‐specific data that might inform on treatment success, potential failure, and off‐target toxicities. Herein, an Aptamer A3 by Cell‐SELEX (systematic evolution of ligands by exponential enrichment) is generated, which can bind with CAR‐T cells with nanomolar affinity. After CAR‐T cells are injected into Nalm6 xenograft tumor model mice through tail vein, Cy5‐labeled A3 is injected into mice for fluorescence time‐delay imaging in vivo. The fluorescence signal produced by the Cy5‐labeled A3 is accumulated in the tumor area and reached its maximum at day 14. Moreover, A3 could enrich CAR‐T cells in mixed cell populations in a traceless way. A3 is screened for CAR‐T cells imaging and CAR‐T cells enrichment, which may provide assistance for the evaluation of CAR‐T cells efficacy and the manufacture of CAR‐T cells. Overall, this research shows that A3 enabled repeated, sensitive, and specific assessment of the infused CAR‐T cells in vivo. The screened aptamer will have broad applications for tracking CAR‐T cells in patients, providing insights into treatment success, potential failure, and off‐target toxicities.https://doi.org/10.1002/advs.202506746CAR‐T cellsDNA aptamersenrichmentfluorescence imagingtracking |
| spellingShingle | Minghui Chen Pengzhao Chang Zhen Zhang Dan Liu Rui Hou Ming Shi Jingjing Li Kai Xu Junnian Zheng Development of a DNA Aptamer‐Based Approach to Noninvasively Image CAR‐T Cells In Vivo and Traceless Enrichment In Vitro Advanced Science CAR‐T cells DNA aptamers enrichment fluorescence imaging tracking |
| title | Development of a DNA Aptamer‐Based Approach to Noninvasively Image CAR‐T Cells In Vivo and Traceless Enrichment In Vitro |
| title_full | Development of a DNA Aptamer‐Based Approach to Noninvasively Image CAR‐T Cells In Vivo and Traceless Enrichment In Vitro |
| title_fullStr | Development of a DNA Aptamer‐Based Approach to Noninvasively Image CAR‐T Cells In Vivo and Traceless Enrichment In Vitro |
| title_full_unstemmed | Development of a DNA Aptamer‐Based Approach to Noninvasively Image CAR‐T Cells In Vivo and Traceless Enrichment In Vitro |
| title_short | Development of a DNA Aptamer‐Based Approach to Noninvasively Image CAR‐T Cells In Vivo and Traceless Enrichment In Vitro |
| title_sort | development of a dna aptamer based approach to noninvasively image car t cells in vivo and traceless enrichment in vitro |
| topic | CAR‐T cells DNA aptamers enrichment fluorescence imaging tracking |
| url | https://doi.org/10.1002/advs.202506746 |
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