Cloning and sequence of GHRH gene of porcine hypothalamus
Porcine ds RNA was extracted from hypothalamus of finishing pig (Duroc × Yorkshire × Lan-drace). A pair or primers was designed according to the GHRH gene sequence reported previously. A cDNA fragment in length 132 bp was synthesized and amplified by RT-PCR. The PCR product was cloned into pGEM-T Ea...
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Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Zhejiang University Press
2000-05-01
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Series: | 浙江大学学报. 农业与生命科学版 |
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Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2000.03.0277 |
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