Cloning and sequence of GHRH gene of porcine hypothalamus

Porcine ds RNA was extracted from hypothalamus of finishing pig (Duroc × Yorkshire × Lan-drace). A pair or primers was designed according to the GHRH gene sequence reported previously. A cDNA fragment in length 132 bp was synthesized and amplified by RT-PCR. The PCR product was cloned into pGEM-T Ea...

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Bibliographic Details
Main Authors: WANG Yi-zhen, YAN Xin-chun, YU Dong-you
Format: Article
Language:English
Published: Zhejiang University Press 2000-05-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/1008-9209.2000.03.0277
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