Optimization of mammosphere formation assay for quantification of il6-induced stemness in differentiated breast cancer cells

Optimization of mammosphere formation assay for quantification of il6-induced stemness in differentiated breast cancer cells

The aim of the study was mammosphere assay optimization for quantifcation of IL6-induced stemness in differentiated (СD44– ) T47D breast cancer cells.Material and Methods. The effect of three commonly used cell-detaching methods (TrypLE, accutase, cell scrapper) at various confuence (40–50 % and 70–...

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Main Authors: K. V. Nevskaya, L. V. Efimova, P. V. Korda, D. S. Dolgasheva, M. K. Ibragimova, M. E. Menyailo, E. V. Udut, N. V. Litviakov, A. G. Pershina
Format: Article
Language:Russian
Published: Russian Academy of Sciences, Tomsk National Research Medical Center 2023-01-01
Series:Сибирский онкологический журнал
Subjects:
breast cancer
mammosphere formation assay
il6
stemness
Online Access:https://www.siboncoj.ru/jour/article/view/2375
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https://www.siboncoj.ru/jour/article/view/2375

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