Plant expression vector's construction of VP2 gene of infectious bursal disease virus
The VP2 gene of infectious bursal disease virus (IBDV, HZ96 strain) was amplified by polymerase chain reaction with special primers containing Kpn Ⅰ and Sal Ⅰ. The purified PCR product was digested by restricted enzymes Kpn Ⅰ and Sal Ⅰ. The digested and purified VP2 gene of infectious bursal disease...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2003-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2003.06.0644 |
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| Summary: | The VP2 gene of infectious bursal disease virus (IBDV, HZ96 strain) was amplified by polymerase chain reaction with special primers containing Kpn Ⅰ and Sal Ⅰ. The purified PCR product was digested by restricted enzymes Kpn Ⅰ and Sal Ⅰ. The digested and purified VP2 gene of infectious bursal disease virus was cloned into plant expression vector Cambia1301 that contains rice endosperm-specific promoter GT1. The result shows that Cambia1301 with VP2 gene was successfully transferred into Agrobacterium tumefaciens (Aotumefaciens) by method of triparental mating. |
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| ISSN: | 1008-9209 2097-5155 |