Mapping QTLs for Stripe Rust Resistance and Agronomic Traits in Chinese Winter Wheat Lantian 31 Using 15K SNP Array

Mapping QTLs for Stripe Rust Resistance and Agronomic Traits in Chinese Winter Wheat Lantian 31 Using 15K SNP Array

Wheat stripe rust (<i>Puccinia striiformis</i> f. sp. <i>tritici</i>, <i>Pst</i>) resistance and agronomic traits are crucial determinants of wheat yield. Elucidating the quantitative trait loci (QTLs) associated with these essential traits can furnish valuable ge...

Full description

Saved in:
Bibliographic Details
Main Authors: Xin Li, Wenjing Tan, Junming Feng, Qiong Yan, Ran Tian, Qilin Chen, Qin Li, Shengfu Zhong, Suizhuang Yang, Chongjing Xia, Xinli Zhou
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Agriculture
Subjects:
adult-plant resistance (APR)
all-stage resistance (ASR)
genetic map
interval composite interval mapping (ICIM)
recombinant inbred line (RIL)
Online Access:https://www.mdpi.com/2077-0472/15/13/1444
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Wheat stripe rust (<i>Puccinia striiformis</i> f. sp. <i>tritici</i>, <i>Pst</i>) resistance and agronomic traits are crucial determinants of wheat yield. Elucidating the quantitative trait loci (QTLs) associated with these essential traits can furnish valuable genetic resources for improving both the yield potential and disease resistance in wheat. Lantian 31 is an excellent Chinese winter wheat cultivar; multi-environment phenotyping across three ecological regions (2022–2024) confirmed stable adult-plant resistance (IT 1–2; DS < 30%) against predominant Chinese <i>Pst</i> races (CYR31–CYR34), alongside superior thousand-kernel weight (TKW) and kernel morphology. Here, we dissected the genetic architecture of these traits using a total of 234 recombinant inbred lines (RILs) derived from a cross between Lantian 31 and the susceptible cultivar Avocet S (AvS). Genotyping with a 15K SNP array, complemented by 660K SNP-derived KASP and SSR markers, identified four stable QTLs for stripe rust resistance (<i>QYrlt.swust-1B</i>, -<i>1D</i>, -<i>2D</i>, -<i>6B</i>) and eight QTLs governing plant height (PH), spike length (SL), and kernel traits. Notably, <i>QYrlt.swust-1B</i> (1BL; 29.9% phenotypic variance) likely represents the pleiotropic <i>Yr29</i>/<i>Lr46</i> locus, while <i>QYrlt.swust-1D</i> (1DL; 22.9% variance) is the first reported APR locus on chromosome 1DL. A pleiotropic cluster on 1B (670.4–689.9 Mb) concurrently enhanced the TKW and the kernel width and area, demonstrating Lantian 31’s dual utility as a resistance and yield donor. The integrated genotyping pipeline—combining 15K SNP discovery, 660K SNP fine-mapping, and KASP validation—precisely delimited <i>QYrlt.swust-1B</i> to a 1.5 Mb interval, offering a cost-effective model for QTL resolution in common wheat. This work provides breeder-friendly markers and a genetic roadmap for pyramiding durable resistance and yield traits in wheat breeding programs.
ISSN:2077-0472

Similar Items