Estimation of stability of DNA-containing vegetable samples stored under the accelerated DNA degradation
There has been carried out an estimation of stability of DNA-containing plant samples stored under the conditions that model accelerated DNA degradation. The research material were the lines H. annuus (“VIR 114”, “VIR 116”) and wild-growing forms of sunflower H. decapetalus, H. petiolaris, H. rigidu...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
Federal State Budgetary Scientific Institution “Agricultural Research Center “Donskoy”"
2020-04-01
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| Series: | Зерновое хозяйство России |
| Subjects: | |
| Online Access: | https://www.zhros.online/jour/article/view/871 |
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| Summary: | There has been carried out an estimation of stability of DNA-containing plant samples stored under the conditions that model accelerated DNA degradation. The research material were the lines H. annuus (“VIR 114”, “VIR 116”) and wild-growing forms of sunflower H. decapetalus, H. petiolaris, H. rigidus taken from the collection of the N. I. Vavilov All-Russian Institute of Plant Genetic Resources. On average, the stability level of long DNA fragments for the nuclear, mitochondrial, and chloroplast genomes was 76.9%, 87.5%, and 98.3% respectively compared with the control. When assessing the stability of short DNA fragments, it was shown that on average, the number of nuclear, mitochondrial, and chloroplast DNA was 89.5%, 96.0%, and 97.7% respectively compared with the control. |
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| ISSN: | 2079-8725 2079-8733 |