Angiogenic potential of circulating peripheral blood neutrophils in kidney cancer
The role of neutrophils in kidney cancer is currently being studied. Their role in carcinogenesis is ambiguous. As one of the most abundant blood leukocytes, neutrophils play an important role in cancer progression through multiple mechanisms, including promotion of angiogenesis, immunosuppression,...
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| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2023-06-01
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| Series: | Медицинская иммунология |
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| Online Access: | https://www.mimmun.ru/mimmun/article/view/2678 |
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| author | I. R. Myagdieva T. V. Abakumova D. R. Dolgova O. U. Gorshkov T. P. Gening |
| author_facet | I. R. Myagdieva T. V. Abakumova D. R. Dolgova O. U. Gorshkov T. P. Gening |
| author_sort | I. R. Myagdieva |
| collection | DOAJ |
| description | The role of neutrophils in kidney cancer is currently being studied. Their role in carcinogenesis is ambiguous. As one of the most abundant blood leukocytes, neutrophils play an important role in cancer progression through multiple mechanisms, including promotion of angiogenesis, immunosuppression, and cancer metastasis. Neutrophils synthesize and release pro-angiogenic factors that are able to directly or indirectly stimulate the growth and migration of endothelial cells, which in turn causes the formation of new blood vessels from pre-existing ones. The production of various factors by neutrophils, including proangiogenic ones, is mediated by the expression of the genes of these molecules. Functional heterogeneity is characterized by differences in neutrophil gene expression patterns. The aim of this study was to evaluate the angiogenic potential of circulating neutrophils in kidney cancer. The object of the study were blood neutrophils of patients with verified clear cell kidney cancer at stage I (T1N0M0G1, n = 28, median age 60), stage II (T2N0M0G2, n = 15, median age 61) and stage III (T3N0M0G2, n = 15, median age 63) before surgery. The control group consisted of apparently healthy donors (n = 15, median age 54). Serum levels of IL-8 and VEGF-A were assessed by enzyme immunoassay. Expression of the CXCL8 and VEGF-A genes in circulating neutrophils was determined by reverse transcription quantitative PCR. As a result of our study, an increase in the level of IL-8 and VEGF-A in the blood serum of patients with kidney cancer in all studied groups compared with the control group was revealed. We observed a direct correlation between serum levels of IL-8 and VEGF-A in patients with kidney cancer (r = 0.429; p = 0.016), which confirms the relationship of these angiogenic factors. A significant increase in CXCL8 gene expression by circulating neutrophils was found in patients on II (2.91, Q0.25-Q0.75: (1.296-4.99), p = 0.02) and III (1.93, Q0.25-Q0.75: (0.755-11.36, p = 0.014) stages of kidney cancer compared with the control group (1.50, Q0.25-Q0.75: (0.80-4.05)). However, VEGF-A gene expression by circulating neutrophils did not differ from those in the control group. Blood neutrophils in kidney cancer exercise their angiogenic potential through the production of IL-8. |
| format | Article |
| id | doaj-art-72744e94e710461fa55f513a56e21f8c |
| institution | Matheson Library |
| issn | 1563-0625 2313-741X |
| language | Russian |
| publishDate | 2023-06-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj-art-72744e94e710461fa55f513a56e21f8c2025-08-04T14:30:42ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2023-06-012551123112810.15789/1563-0625-APO-26781764Angiogenic potential of circulating peripheral blood neutrophils in kidney cancerI. R. Myagdieva0T. V. Abakumova1D. R. Dolgova2O. U. Gorshkov3T. P. Gening4Ulyanovsk State UniversityUlyanovsk State UniversityUlyanovsk State UniversityRegional Clinical Oncologic CenterUlyanovsk State UniversityThe role of neutrophils in kidney cancer is currently being studied. Their role in carcinogenesis is ambiguous. As one of the most abundant blood leukocytes, neutrophils play an important role in cancer progression through multiple mechanisms, including promotion of angiogenesis, immunosuppression, and cancer metastasis. Neutrophils synthesize and release pro-angiogenic factors that are able to directly or indirectly stimulate the growth and migration of endothelial cells, which in turn causes the formation of new blood vessels from pre-existing ones. The production of various factors by neutrophils, including proangiogenic ones, is mediated by the expression of the genes of these molecules. Functional heterogeneity is characterized by differences in neutrophil gene expression patterns. The aim of this study was to evaluate the angiogenic potential of circulating neutrophils in kidney cancer. The object of the study were blood neutrophils of patients with verified clear cell kidney cancer at stage I (T1N0M0G1, n = 28, median age 60), stage II (T2N0M0G2, n = 15, median age 61) and stage III (T3N0M0G2, n = 15, median age 63) before surgery. The control group consisted of apparently healthy donors (n = 15, median age 54). Serum levels of IL-8 and VEGF-A were assessed by enzyme immunoassay. Expression of the CXCL8 and VEGF-A genes in circulating neutrophils was determined by reverse transcription quantitative PCR. As a result of our study, an increase in the level of IL-8 and VEGF-A in the blood serum of patients with kidney cancer in all studied groups compared with the control group was revealed. We observed a direct correlation between serum levels of IL-8 and VEGF-A in patients with kidney cancer (r = 0.429; p = 0.016), which confirms the relationship of these angiogenic factors. A significant increase in CXCL8 gene expression by circulating neutrophils was found in patients on II (2.91, Q0.25-Q0.75: (1.296-4.99), p = 0.02) and III (1.93, Q0.25-Q0.75: (0.755-11.36, p = 0.014) stages of kidney cancer compared with the control group (1.50, Q0.25-Q0.75: (0.80-4.05)). However, VEGF-A gene expression by circulating neutrophils did not differ from those in the control group. Blood neutrophils in kidney cancer exercise their angiogenic potential through the production of IL-8.https://www.mimmun.ru/mimmun/article/view/2678neutrophilsangiogenesisvegf-ail-8neutrophil phenotypekidney cancer |
| spellingShingle | I. R. Myagdieva T. V. Abakumova D. R. Dolgova O. U. Gorshkov T. P. Gening Angiogenic potential of circulating peripheral blood neutrophils in kidney cancer Медицинская иммунология neutrophils angiogenesis vegf-a il-8 neutrophil phenotype kidney cancer |
| title | Angiogenic potential of circulating peripheral blood neutrophils in kidney cancer |
| title_full | Angiogenic potential of circulating peripheral blood neutrophils in kidney cancer |
| title_fullStr | Angiogenic potential of circulating peripheral blood neutrophils in kidney cancer |
| title_full_unstemmed | Angiogenic potential of circulating peripheral blood neutrophils in kidney cancer |
| title_short | Angiogenic potential of circulating peripheral blood neutrophils in kidney cancer |
| title_sort | angiogenic potential of circulating peripheral blood neutrophils in kidney cancer |
| topic | neutrophils angiogenesis vegf-a il-8 neutrophil phenotype kidney cancer |
| url | https://www.mimmun.ru/mimmun/article/view/2678 |
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