Phenotype Characterization of Beta-Lactamase Producing Enterobacteriaceae in the Intensive Care Unit (ICU) of Cipto Mangunkusumo Hospital in 2011
Aim: the goal of this study is to understand the phenotype characteristic of beta-lactamase enzymes producing Enterobacteriaceae, such as ESBL, AmpC, and carbapenemase. Methods: three different methods are performed to confirm those phenotypic characteristics, namely double disk diffusion method to...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Interna Publishing
2016-05-01
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| Series: | Acta Medica Indonesiana |
| Online Access: | https://actamedindones.org/index.php/ijim/article/view/115 |
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| Summary: | Aim: the goal of this study is to understand the phenotype characteristic of beta-lactamase enzymes producing Enterobacteriaceae, such as ESBL, AmpC, and carbapenemase. Methods: three different methods are performed to confirm those phenotypic characteristics, namely double disk diffusion method to confirm ESBL, AmpC disk test (cefoxitin-based) to confirm AmpC, and modified Hodge test to confirm carbapenemase. Results: using double disk diffusion method, we found 58.42% isolates are ESBL-producing, whereas the outcomes of AmpC disk test shows 1.98% are AmpC-producing. By conducting modified Hodge test (MHT), 27.59% isolates are confirmed as carbapenemase-producing bacteria. Conclusion: this study confirmed the prevalence of beta-lactamase producing Klebsiella pneumoniae is very high. Nevertheless, AmpC and carbapenemase should not be ignored despite their low prevalence.
Key words: Enterobacteriaceae, antibiotic resistance, ESBL, AmpC, carbapenemase.
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| ISSN: | 0125-9326 2338-2732 |