Combining Phytate/Ca2+ Fractionation with Trichloroacetic Acid/Acetone Precipitation Improved Separation of Low-Abundant Proteins of Wheat (Triticum aestivum L.) Leaf for Proteomic Analysis

Proteomic assessment of low-abundance leaf proteins is hindered by the large quantity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) present within plant leaf tissues. In the present study, total proteins were extracted from wheat (Triticum aestivum L.) leaves by a conventional trichlo...

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Auteurs principaux: Muhammad ARF Sultan, Hui LIU, Yu-Feng CHENG, Pei-pei ZHANG, Hui-xian ZHAO
Format: Article
Langue:anglais
Publié: KeAi Communications Co., Ltd. 2013-07-01
Collection:Journal of Integrative Agriculture
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Accès en ligne:http://www.sciencedirect.com/science/article/pii/S2095311913603445
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