Protocol for measuring D-amino acid transport in mammalian cells using a fluorescent biosensor

Summary: The movement of amino acids into cells and organelles is facilitated by transporter proteins, which support homeostasis, metabolism, and disease progression. Here, we present a protocol to visualize amino acid transport in mammalian cells in real time using an enzyme-based biosensor. We pro...

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Bibliographic Details
Main Authors: Danisha Johal, Keeley G. Hewton, Sadie J. Gale, Amritpal S. Johal, Seth J. Parker
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725003351
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