Protocol for measuring D-amino acid transport in mammalian cells using a fluorescent biosensor
Summary: The movement of amino acids into cells and organelles is facilitated by transporter proteins, which support homeostasis, metabolism, and disease progression. Here, we present a protocol to visualize amino acid transport in mammalian cells in real time using an enzyme-based biosensor. We pro...
Saved in:
Main Authors: | Danisha Johal, Keeley G. Hewton, Sadie J. Gale, Amritpal S. Johal, Seth J. Parker |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2025-09-01
|
Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725003351 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
L-Lysine-Linked Modular Fluorescent Cholesteryl Mimics: Biophysical Properties, Molecular Interactions, and Cellular Applications
by: Nicholas McInchak, et al.
Published: (2025-05-01) -
Protocol for assembling photostable fluorescence-labeled tiny DNA origami blocks to observe kinesin cooperation
by: Tomoki Kita, et al.
Published: (2025-09-01) -
Protocol to study adenosine release in the adult mouse hippocampus using a genetically encoded sensor, 2-photon live imaging, and fiber photometry
by: Coralie Berthoux, et al.
Published: (2025-09-01) -
Protocol to detect m22G modification on single tRNA iso-decoders by immuno-northern blotting
by: Sseu-Pei Hwang, et al.
Published: (2025-09-01) -
Atomic force microscopy of erythrocytes of patients with different severity of pancreatitis
by: R. A. Pakhomova, et al.
Published: (2023-08-01)