Protocol to study the contribution of tendon lineage in the development of mouse temporomandibular joint condylar cartilage using 3D visualization
Summary: Tendon cells have been shown to play a critical role during the postnatal growth of the temporomandibular joint (TMJ) condyle by transdifferentiating into chondrocytes. Here, we present a protocol for tracking the tendon lineage of ScxCreERT2; R26RtdTomato; 2.3Col1a1-GFP mice in vivo. We de...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725003399 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839634666126049280 |
|---|---|
| author | Paria Dehghanian Vishal Gokani Matthew J. Kesterke Yan Jing Chi Ma |
| author_facet | Paria Dehghanian Vishal Gokani Matthew J. Kesterke Yan Jing Chi Ma |
| author_sort | Paria Dehghanian |
| collection | DOAJ |
| description | Summary: Tendon cells have been shown to play a critical role during the postnatal growth of the temporomandibular joint (TMJ) condyle by transdifferentiating into chondrocytes. Here, we present a protocol for tracking the tendon lineage of ScxCreERT2; R26RtdTomato; 2.3Col1a1-GFP mice in vivo. We describe steps for sample preparation, tissue clearing, confocal microscopy, and artificial intelligence-based analysis, which are also applicable to other mineralized tissues. This protocol enables the study of the contribution of tendon cells to condylar chondrogenesis through 3D visualization.For complete details on the use and execution of this protocol, please refer to Yi et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-68d6df41b4e9486c90edfe4f66e2912d |
| institution | Matheson Library |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-68d6df41b4e9486c90edfe4f66e2912d2025-07-10T04:35:08ZengElsevierSTAR Protocols2666-16672025-09-0163103933Protocol to study the contribution of tendon lineage in the development of mouse temporomandibular joint condylar cartilage using 3D visualizationParia Dehghanian0Vishal Gokani1Matthew J. Kesterke2Yan Jing3Chi Ma4Department of Orthodontics, Texas A&M University College of Dentistry, Dallas, TX 75246, USACenter of Excellence in Hip, Scottish Rite for Children, Dallas, TX 75219, USA; Department of Orthopedic Surgery, University of Texas Southwestern Medical Center, Dallas, TX 75390, USADepartment of Orthodontics, Texas A&M University College of Dentistry, Dallas, TX 75246, USADepartment of Orthodontics, Texas A&M University College of Dentistry, Dallas, TX 75246, USA; Corresponding authorDepartment of Orthodontics, Texas A&M University College of Dentistry, Dallas, TX 75246, USA; Center of Excellence in Hip, Scottish Rite for Children, Dallas, TX 75219, USA; Department of Orthopedic Surgery, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Corresponding authorSummary: Tendon cells have been shown to play a critical role during the postnatal growth of the temporomandibular joint (TMJ) condyle by transdifferentiating into chondrocytes. Here, we present a protocol for tracking the tendon lineage of ScxCreERT2; R26RtdTomato; 2.3Col1a1-GFP mice in vivo. We describe steps for sample preparation, tissue clearing, confocal microscopy, and artificial intelligence-based analysis, which are also applicable to other mineralized tissues. This protocol enables the study of the contribution of tendon cells to condylar chondrogenesis through 3D visualization.For complete details on the use and execution of this protocol, please refer to Yi et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725003399Cell BiologyDevelopmental biologyMicroscopyComputer sciences |
| spellingShingle | Paria Dehghanian Vishal Gokani Matthew J. Kesterke Yan Jing Chi Ma Protocol to study the contribution of tendon lineage in the development of mouse temporomandibular joint condylar cartilage using 3D visualization STAR Protocols Cell Biology Developmental biology Microscopy Computer sciences |
| title | Protocol to study the contribution of tendon lineage in the development of mouse temporomandibular joint condylar cartilage using 3D visualization |
| title_full | Protocol to study the contribution of tendon lineage in the development of mouse temporomandibular joint condylar cartilage using 3D visualization |
| title_fullStr | Protocol to study the contribution of tendon lineage in the development of mouse temporomandibular joint condylar cartilage using 3D visualization |
| title_full_unstemmed | Protocol to study the contribution of tendon lineage in the development of mouse temporomandibular joint condylar cartilage using 3D visualization |
| title_short | Protocol to study the contribution of tendon lineage in the development of mouse temporomandibular joint condylar cartilage using 3D visualization |
| title_sort | protocol to study the contribution of tendon lineage in the development of mouse temporomandibular joint condylar cartilage using 3d visualization |
| topic | Cell Biology Developmental biology Microscopy Computer sciences |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725003399 |
| work_keys_str_mv | AT pariadehghanian protocoltostudythecontributionoftendonlineageinthedevelopmentofmousetemporomandibularjointcondylarcartilageusing3dvisualization AT vishalgokani protocoltostudythecontributionoftendonlineageinthedevelopmentofmousetemporomandibularjointcondylarcartilageusing3dvisualization AT matthewjkesterke protocoltostudythecontributionoftendonlineageinthedevelopmentofmousetemporomandibularjointcondylarcartilageusing3dvisualization AT yanjing protocoltostudythecontributionoftendonlineageinthedevelopmentofmousetemporomandibularjointcondylarcartilageusing3dvisualization AT chima protocoltostudythecontributionoftendonlineageinthedevelopmentofmousetemporomandibularjointcondylarcartilageusing3dvisualization |