Differentiation and histological analysis of primary human bronchial epithelial cells cultured on transwell membranes using air-liquid interface culture
This protocol describes a reproducible and optimized workflow for the differentiation and histological analysis of primary normal human bronchial epithelial cells (NHBECs) cultured on Transwell membranes using air-liquid interface (ALI) culture. The method begins with expansion of early-passage NHBE...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-12-01
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| Series: | MethodsX |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016125003371 |
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