In vivo proximity-labeling with miniTurboID to screen for protein-protein interactions in the filamentous ascomycete Sordaria macrospora
The Biotin Identification (BioID) method applies proximity-dependent labeling of co-localizing proteins to screen for protein-protein interactions in vivo. Therefore, the protein of interest (POI) is fused to a promiscuous biotin ligase. This ligase covalently biotinylates proximal proteins, which a...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
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| Series: | MethodsX |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016125001979 |
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