Improving enzymatic properties of BlTDH from Bacillus licheniformis through site-directed mutagenesis

L-threonine dehydrogenase (TDH) is a rate-limiting enzyme in the biosynthesis pathway of 2,5-dimethylpyrazine and 2,3,5-trimethylpyrazine, which are widely used food additives. However, natural TDH enzymes suffer from low catalytic activity and poor environmental adaptability, limiting their industr...

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Bibliographic Details
Main Authors: Xun Liu, Hongyi Gu, Han Li, Shuanglian Chen, Zhen Tang, Wenli Quan
Format: Article
Language:English
Published: Elsevier 2025-12-01
Series:Food Chemistry: Molecular Sciences
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666566225000334
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