Characterization of Cellulase from the Rumen Metagenome and Evaluation of its Hydrolytic Potential for Agricultural Wastes

Characterization of Cellulase from the Rumen Metagenome and Evaluation of its Hydrolytic Potential for Agricultural Wastes

The cellulase RuCel224, derived from the rumen metagenome, was successfully expressed in Escherichia coli BL21(DE3), with a molecular weight of ~43 kDa, as confirmed by sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. Substrate specificity assays revealed the highest activity against...

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Bibliographic Details
Main Authors: Meng Ouyang, Chanjuan Liu, Ping Sheng, Qinghua Qiu, Kehui Ouyang, Yanjiao Li, Yitian Zang, Xianghui Zhao
Format: Article
Language:English
Published: North Carolina State University 2025-06-01
Series:BioResources
Subjects:
rumen metagenome
cellulases
enzymatic properties
agricultural wastes
Online Access:https://ojs.bioresources.com/index.php/BRJ/article/view/24352
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