A highly efficient SFFV promoter-driven mCherry-GFP-LC3B dual-fluorescence system for autophagy monitoring in erythroid cells
Objective To develop a modified lentiviral expression system of mCherry-GFP-LC3B, driven by the hematopoietic-specific SFFV (spleen focus-forming virus) promoter, in order to perform an efficient and real-time monitoring of autophagy flux with in terminally differentiated erythroid cells. Methods Th...
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| Main Author: | |
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| Format: | Article |
| Language: | Chinese |
| Published: |
Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2025-07-01
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| Series: | Jichu yixue yu linchuang |
| Subjects: | |
| Online Access: | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-7-866.pdf |
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