Construction of an eukaryotic expression vector containing IBDV VP2/4/3 and ChIL-2 fusion gene and their expression in Vero cells
Recombinant eukaryotic expression vectors containing fusion genes of infectious bursal disease virus (IBDV) polyprotein (VP2/4/3) gene and chicken interleukin 2 (ChIL-2) gene were constructed using the technique of splicing by overlapping extension (SOEing) and three times PCR. Fusion gene fragments...
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Main Authors: | WAN Wang-jun, XIE Rong-hui, LI Long, XU Jian, YU Lian |
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Format: | Article |
Language: | English |
Published: |
Zhejiang University Press
2005-05-01
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Series: | 浙江大学学报. 农业与生命科学版 |
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Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2005.03.0255 |
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