Construction of an eukaryotic expression vector containing IBDV VP2/4/3 and ChIL-2 fusion gene and their expression in Vero cells

Recombinant eukaryotic expression vectors containing fusion genes of infectious bursal disease virus (IBDV) polyprotein (VP2/4/3) gene and chicken interleukin 2 (ChIL-2) gene were constructed using the technique of splicing by overlapping extension (SOEing) and three times PCR. Fusion gene fragments...

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Bibliographic Details
Main Authors: WAN Wang-jun, XIE Rong-hui, LI Long, XU Jian, YU Lian
Format: Article
Language:English
Published: Zhejiang University Press 2005-05-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/1008-9209.2005.03.0255
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