Splice-site mutation enhances the efficiency of CRISPR-mediated gene knockout and new germplasm creation in fish
The knockout efficiency of the CRISPR/Cas9 system largely relies on the frameshift mutation caused by random insertions or deletions at target site of coding DNA sequence (CDS). Splice site (SS) mutations result in mRNA mis-splicing, making gRNAs targeting SS more likely to produce null mutations an...
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Main Authors: | , , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2025-09-01
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Series: | Aquaculture Reports |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425003813 |
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