A practical protocol for correlative confocal fluorescence and transmission electron microscopy characterization of extracellular vesicles

ABSTRACT Extracellular vesicles (EVs) have gained considerable attention in fungal biology research. However, imaging these membrane-bound particles presents challenges due to their nanoscopic size (typically <200 nm), which exceeds the resolution limit of conventional diffraction-limited laser s...

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Bibliographic Details
Main Authors: Juan M. Martínez-Andrade, Daniel Salgado-Bautista, Kendra Ramirez-Acosta, Ruben Darío Cadena-Nava, Meritxell Riquelme
Format: Article
Language:English
Published: American Society for Microbiology 2025-07-01
Series:Microbiology Spectrum
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Online Access:https://journals.asm.org/doi/10.1128/spectrum.03026-24
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