A Method of Well-Spread Pachytene Chromosome Preparations for Plant Species with Large Genomes Suitable for the Immunolocalization of Meiotic Proteins

Well-spread pachytene chromosomes are critical for studying the location of meiotic proteins along individual chromosomes. However, producing good spreads in species with large genomes is challenging due to the tangling of pachytene chromosomes. Existing protocols often fail to achieve proper separa...

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Bibliographic Details
Main Authors: Natalya Kudryavtseva, Aleksey Ermolaev, Ludmila Khrustaleva
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Methods and Protocols
Subjects:
Online Access:https://www.mdpi.com/2409-9279/8/3/54
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