A Method of Well-Spread Pachytene Chromosome Preparations for Plant Species with Large Genomes Suitable for the Immunolocalization of Meiotic Proteins
Well-spread pachytene chromosomes are critical for studying the location of meiotic proteins along individual chromosomes. However, producing good spreads in species with large genomes is challenging due to the tangling of pachytene chromosomes. Existing protocols often fail to achieve proper separa...
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Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2025-05-01
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Series: | Methods and Protocols |
Subjects: | |
Online Access: | https://www.mdpi.com/2409-9279/8/3/54 |
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