Rezultaty - PCR detection
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161
Detection a New Antiseptic Resistant Variant of qac Gene in Some Multi Drug Resistant Staphylococcus aureus Isolated from Different Clinical Sources
Wydane 2019-09-01“…Simultaneous discrimination of antiseptic coding genes (qacA/B, smr and norA)as well as nuc and mecA genes among multidrug resistantS.aureus(MRSA) isolates was preformed using multiplex PCR assay , 61 isolatesamong 65 were positive tonucand mecA genes, 58 of them were positive to norA, 14 of them were positive to qacA/B and only two were positive to smr. …”The increasing use of antiseptic compounds creates selective pressure cause emergence of antiseptic resistance among Staphylococcus aureus .Resistance mechanism of antiseptic is driven mainly by multi drug resistant (MDR) efflux protein.Sixty five isolates of S.aureuswere collected from different cl...
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162
Preparation and detection application of monoclonal antibodies against Potato virus Y
Wydane 2016-09-01“…The detection results of serological assays were the same as the result of RT-PCR, and 20 of the 30 potato field samples were PVY-positive. …”Potato virus Y (PVY) is one of the most important potato viruses causing serious economic loss in potato industry worldwide. Establishment of a sensitive and specific virus detection technique is the key to prevent and control PVY.To provide material and technical support for the diagnosis, scientif...
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163
Real-Time Diagnostics of Anaplasmosis in Cattle by PCR Method
Wydane 2019-10-01“…Results and discussion. PCR method has been developed in real time mode to detect A. marginale anaplasmosis agent in cattle. …”The purpose of the research is real-time development of PCR method for diagnostics of anaplasmosis in cattle. Materials and methods. For real time development of primers and fluorescence-labeled probe for PCR msp1α gene sequences 57 isolates Anaplasma marginale available on database Genbank (http://...
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164
Stool-based PCR test for colorectal cancer screening
Wydane 2014-12-01Dokumenty pełnotekstoweThe research represents working out of non-invasive colorectal cancer screening based on DNA integrity testing of patient's fecal. Polymerase chain reaction method and the following polyacrylamide gel electrophoresis in fecal of patient with colorectal cancer detected long DNA fragments which s...
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165
BREAST CANCER TYPING USING RT-PCR ASSAY
Wydane 2019-11-01“…Surgical specimens were studied using the standard immunohistochemistry (IHC) and RT-PCR for detecting expression of 24 genes.Results. …”Introduction. Adjuvant systemic therapy remains one of the main options for treating breast cancer. Results of standard immunohistochemical studies are not always a criterion for selecting systemic therapy. Nowadays, multigene expression analysis is actively used to predict the response to chemother...
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166
Optimization of a Method for Detecting Single copies of Hepatitis B Virus DNA using CRISPR/Cas systems
Wydane 2025-01-01Hasła przedmiotowe: Dokumenty pełnotekstoweRelevance. Hepatitis B virus (HBV) is the etiologic agent of acute and chronic hepatitis B in humans. WHO recommends the use of sensitive laboratory assays based on nucleic acid amplification methods to detect HBV DNA. A method for detecting single copies of hepatitis B virus DNA using CRISPR/Cas sy...
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167
Detection of High-Risk Human Papillomavirus in Bladder Cancer: An Exploratory Study from a UK-Based Population
Wydane 2025-06-01“…<b>Methods</b>: A total of 55 fresh bladder specimens, including 4 benign and 51 malignant samples, were analysed using polymerase chain reaction (PCR) and Sanger sequencing to detect 12 HR-HPV types. …”<b>Background/Objectives</b>: Human papillomavirus (HPV) is a prevalent sexually transmitted infection globally and is linked to the development of various cancers. While several international studies have investigated the incidence of high-risk HPV (HR-HPV) in bladder cancers, no such r...
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168
COVID19 detection in appendix of acute appendicitis in a child: a case report and review of literature
Wydane 2023-03-01Dokumenty pełnotekstoweAbstract Background Gastrointestinal symptoms are one of the most common presentations of Coronavirus disease-19 (COVID-19), even in children. Higher rates of complicated appendicitis have been demonstrated in the era of the COVID-19 outbreak, and it has been recently suggested that acute appendicit...
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169
First Detection of <i>Rhodococcus equi</i> in a Foal in Bulgaria—A Case Report
Wydane 2025-07-01“…Microbiological tests and PCR confirmed the presence of <i>R. equi</i> carrying the virulence-associated protein A (<i>VapA</i>) gene. …”This case report describes the following scenario: A 56-day-old Arabian filly presented with classical symptoms of respiratory distress and a rapidly deteriorating condition, despite intensive antimicrobial treatment, resulting in death. The post-mortem examination revealed severe bronchopneumonia w...
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170
Assessing the Efciency of Detection of Vibrio cholerae Genetic Determinants Through Waterbody Vibrioflora Monitoring System
Wydane 2021-10-01“…Species-specifc (hlyA, toxR) and serogroup-specifc (wbeT, wbfR) V. cholerae determinants were indicated in PCR with hybridization-fluorescent and electrophoretic detection.Results and discussion. …”Objective of the study was to assess the effectiveness of PCR screening of Vibrio cholerae genetic determinants in samples from surface water reservoirs for optimization of the cholera microbiological monitoring system.Materials and methods. The study was carried out as a part of the vibrioflora monit...
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171
Detection and tissue-localization of Himetobi P virus in Nilaparvata lugens (Stål)
Wydane 2013-07-01“…In phylogenetic analysis based on the viral capsid protein sequences, the 10 virus strains were clustered into four groups with less relevance to the host geographic locations.The virus was not evidently pathogenic to BPH, suggesting that it may be a symbiont of the insect. Real-time PCR detections showed that the relative amount of HiPV in BPH increased gradually along with nymph growth and increased sharply after adult emergence. …”The brown planthopper (BPH), Nilaparvata lugens (Stål), is a serious insect pest of rice plants causing sucking damage and transmitting rice viruses, such as rice ragged stunt virus and rice grassy stunt virus. BPH has caused serious yield losses in Asia in recent decades. Himetobi P virus (HiPV) is...
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172
Genome-Informed Real-Time Polymerase Chain Reaction for Detection of Erwinia pyrifoliae, the Causal Agent of Asian Pear Blight
Wydane 2025-06-01Hasła przedmiotowe: Dokumenty pełnotekstoweErwinia pyrifoliae causes blight symptoms on pears (Pyrus spp.), apple (Malus spp.), and strawberry (Fragaria × ananassa), with up to 40% losses in strawberry observed during a 2013 outbreak in the Netherlands. These commodities are of economic importance in the United States. Introduction and estab...
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173
Development and Testing of a Method for Detecting Lujo Virus RNA by Reverse Transcription and Real Time Polymerase Chain Reaction
Wydane 2021-04-01“…The aim of the study was to develop and assess the efficacy of a method for Lujo virus RNA detection in clinical and biological samples using one-step real-time RT-PCR.Materials and methods. …”The aim of the study was to develop and assess the efficacy of a method for Lujo virus RNA detection in clinical and biological samples using one-step real-time RT-PCR.Materials and methods. In order to select the conservative regions of the genome, we utilized the available in GenBank database Lujo...
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174
Presence and distribution of apple proliferation disease in the Kyustendil region of Bulgaria
Wydane 2025-06-01Hasła przedmiotowe: Dokumenty pełnotekstoweApple proliferation is a significant economic disease affecting cultivated apple trees across Europe. This study examines the presence, distribution, and symptomatic expression of apple proliferation disease among apple cultivars in commercial orchards in the Kyustendil region of Bulgaria. A total...
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175
Enterovirus Detection Trends Based on Respiratory Specimens from a Single Tertiary Hospital in Korea (2018–2024): A Retrospective Study Using Multiplex PCR Data
Wydane 2025-07-01Dokumenty pełnotekstoweEnteroviruses (EVs) cause broad clinical manifestations, particularly in children. Certain serotypes have been implicated in respiratory infections; however, epidemiological studies analyzing EV circulation based on clinical respiratory specimens are limited in Korea. This retrospective study evalua...
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176
A one-step multiplex qPCR assay for simultaneous identification and quantification of Leishmania martiniquensis and Leishmania orientalis/Leishmania chancei and detection and quantification of trypanosomatids in clinical samples
Wydane 2025-01-01“…Here, a one-step multiplex qPCR assay for the simultaneous identification and quantification of L. martiniquensis and L. orientalis/L. chancei and detection and quantification of trypanosomatids was developed using ITS1 as the molecular target and human RNase P as the internal control gene. …”Leishmaniasis is one of the most important zoonotic diseases. Recently, Leishmania (Mundinia) martiniquensis, Leishmania (Mundinia) orientalis, and Leishmania (Mundinia) chancei have been reported as new human pathogens. Trypanosomatids, apart from Leishmania spp., such as Crithidia spp., are also o...
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177
Relationship between Microscopic Agglutination Test and Real-Time PCR Assay for Detection of Seropositivity of Pathogenic <i>Leptospira</i> Infections in Cattle in Chile: A Pilot Study
Wydane 2025-03-01“…Real-time PCR detected <i>Leptospira</i> infection and bacterial renal excretion, thus providing insights into the presence and extent of environmental contamination, whereas the MAT identified seropositivity, antibody titers, and the serogroups associated with infection. …”<div xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" class="section"> <a class="named-anchor" id="d1449481e140"> <!-- named anchor --> </a> <h5 class="section-title...
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178
Molecular and Microscopic Challenges in Detecting <i>Plasmodium cynomolgi</i> Co-Infections with <i>Plasmodium vivax:</i> A Case Report
Wydane 2025-06-01“…The initial diagnoses made by the microscopy and qPCR conducted by the Kota Bharu Public Health Laboratory in Kelantan identified the infection as <i>P. vivax</i>. …”The risk of non-human primate (NHP) malaria transmission to humans is increasing, with <i>Plasmodium knowlesi</i> and <i>Plasmodium cynomolgi</i> emerging as significant zoonotic threats, particularly in Malaysia. While <i>P. knowlesi</i> is well-documented, <i...
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179
Detection of some genes encoding pathogenicity factors in the typical isolates of Escherichia coli
Wydane 2014-09-01Dokumenty pełnotekstoweUsing polymerase chain reaction 316 strains of E. coli (strains with normal enzymatic activity, strains with weak enzymatic activity and strains with hemolytic activity) were examined for the presence of pathogenicity genes. They were isolatedfrom healthy children and children with functional disord...
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180
Detection of bartonella DNA in ixodid ticks, mosquitoes and in patients blood in Novosibirsk region
Wydane 2006-12-01“…Bartonella DNA was detected using nested PCR in ticks Ixodes persulcatus and Dermacentor reticulatus, in mosquitoes Aedes cantans, but not in other mosquito or gnat species. …”Bartonella DNA was detected using nested PCR in ticks Ixodes persulcatus and Dermacentor reticulatus, in mosquitoes Aedes cantans, but not in other mosquito or gnat species. Phylogenic analysis of the PCR product nucleotide sequences proved the infec- tion of arthropod vectors and human blood with B...
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