Rezultaty - PCR detection
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141
Development of a Triplex Real-Time PCR Method for the Simultaneous Detection of Porcine Circovirus 2, 3, and 4 in China Between 2023 and 2024
Wydane 2025-05-01“…Conclusions: The triple qPCR method established in this study provides a rapid, specific, and accurate approach for the detection and differentiation of PCV2, PCV3, and PCV4 genotypes.…”Background: Porcine circovirus disease (PCVD), caused by porcine circovirus (PCV), is a significant swine disease characterized by porcine dermatitis, nephrotic syndrome, and reproductive disorders in sows. Given the overlapping clinical presentations of PCV2, PCV3, and PCV4, a rapid and accurate me...
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142
Development of a Multiplex Real-Time PCR Assay for the Detection of Eight Pathogens Associated with Bovine Respiratory Disease Complex from Clinical Samples
Wydane 2025-07-01“…In this study, a novel multiplex fluorescence-based quantitative polymerase chain reaction (qPCR) assay was developed for the simultaneous detection of eight major pathogens associated with BRDC. …”Bovine respiratory disease complex (BRDC) is one of the primary causes of morbidity, mortality, and economic loss in cattle worldwide. Accurate and rapid identification of causative pathogenic agents is essential for effective disease management and control. In this study, a novel multiplex fluoresc...
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143
Development of a real-time RT-PCR method for the detection of newly emerged highly pathogenic H7N9 influenza viruses
Wydane 2017-09-01“…Importantly, the real-time RT-PCR method exhibited 100% consistency with the virus isolation method in the diagnosis of field samples. …”In 2013, a human influenza outbreak caused by a novel H7N9 virus occurred in China. Recently, the H7N9 virus acquired multiple basic amino acids at its hemagglutinin (HA) cleavage site, leading to the emergence of a highly pathogenic virus. The development of an effective diagnostic method is impera...
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144
Molecular Detection of tox A Gene in Multidrug Resistance Pseudomonas aeruginosa Isolated from Burn Infections
Wydane 2025-04-01Dokumenty pełnotekstoweBackground: Exotoxin A (ExoA) is the most widespread and toxic virulence agent among pathogenic Pseudomonas aeruginosa species that acquire adenosine diphosphate-ribosyltransferase activity belonging to the class of exotoxins secreted by pathogenic bacteria that cause human diseases. Objectives: The...
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145
Comparative genomics provide a rapid detection of Fusarium oxysporum f. sp. conglutinans
Wydane 2016-04-01Hasła przedmiotowe: Dokumenty pełnotekstoweFusarium oxysporum f. sp. conglutinans (Foc) is the causal agent of Fusarium wilt disease of Brassica oleracea. A rapid, accurate, and reliable method to detect and identify plant pathogens is vitally important to integrated disease management. In this study, using a comparative genome analysis amon...
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146
Рroblems of PCR diagnostics OF COVID-19
Wydane 2022-10-01“…This study analyzed the possible causes of false-positive and false-negative PCR results. The correlation of the number of positive results with the dynamics of detection of new cases of COVID-19 in St. …”To date, an adequate and timely assessment of the number of cases is the basis of effective measures aimed at preventing the spread of COVID-19 infection. Real-time reverse transcription polymerase chain reaction (RT-PCR) remains the gold standard for confirming COVID-19.The purpose of the work: to...
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147
A multiplex real-time PCR assay for simultaneous detection of classical swine fever virus, African swine fever virus, and atypical porcine pestivirus
Wydane 2023-02-01“…Twenty-two virus samples were detected by the multiplex real-time PCR, compared with national standard diagnostic and patented method assay for CSF (GB/T 27540–2011), ASF (GB/T 18648–2020), and APPV (CN108611442A), respectively. …”With the implementation of the C-strain vaccine, classical swine fever (CSF) has been under control in China, which is currently in a chronic atypical epidemic situation. African swine fever (ASF) emerged in China in 2018 and spread quickly across the country. It is presently occurring sporadically...
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148
Frequency of SARS-CoV-2 RNA Detection in Foreign Medical Students Vaccined against New Coronavirus Infection
Wydane 2023-03-01“…Results. SARS-CoV-2 RNA was detected in 41 (8.0% [95% CI 5.6÷10.4]) of 510 international students surveyed. …”Relevance. The incidence of the novel coronavirus infection is on the rise worldwide. To effectively combat COVID-19, both vaccination and control of the circulation of SARS-CoV-2 strains among the population and, especially, foreign citizens arriving in Russia from around the world are necessary. A...
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149
Specific Nested PCR for the Detection of 16SrI and 16SrII Group Phytoplasmas Associated with Yellow Leaf Disease of Areca Palm in Hainan, China
Wydane 2025-07-01“…Field validation using leaf samples from symptomatic areca palms confirmed the high specificity and reliability of the new primers in detecting APYL. Compared to conventional universal primers (P1/P7 and R16mF2/R16mR1), this newly developed nested PCR system demonstrated higher specificity, sensitivity, and speed, making it a valuable tool for the early diagnosis and management of YLD in areca palms.…”Yellow leaf disease (YLD), caused by the areca palm yellow leaf phytoplasma (APYL), poses a significant threat to the sustainability of the areca palm industry. Timely and accurate detection is essential for effectively diagnosing and managing this disease. This study developed a novel nested PCR sy...
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150
Development of Amplification Test-Systems for Tularemia Agent Detection
Wydane 2015-06-01Hasła przedmiotowe: Dokumenty pełnotekstoweDeveloped is the method for tularemia microbe DNA detection using PCR with electrophoretic and hybridization-fluorescent registration of results. iglBC genes have been chosen as DNA-matrixes, being species-specific ones for tularemia agent. Based on the results obtained constructed have been prepara...
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151
Molecular detection of oprL gene in Pseudomonas aeruginosa associated with surgical site infections in Bauchi, Nigeria
Wydane 2025-08-01“…Conclusion: The prevalence of Pseudomonas aeruginosa isolates associated with SSIs was 2.0% from the traditional assays. The PCR assay of the isolates detected the oprL gene in all the isolates. …”Background: Pseudomonas aeruginosa (P. aeruginosa) associated with surgical site infections (SSIs) is often multidrug-resistant and associated with delayed wound healing, morbidity, and deaths. Molecular detection of P. aeruginosa is often required for specific diagnosis for optimal care. Although...
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152
COVID-19 patient variables associated with the detection of airborne SARS-CoV-2
Wydane 2025-08-01“…No significant associations were found between airborne SARS-CoV-2 detection and symptoms presence or duration, nor with the results of the most recent positive nasopharyngeal PCR test prior to air sampling. …”Background: Understanding the COVID-19 patient characteristics that impact environmental SARS-CoV-2 load is essential for improving infection risk management. In this study, we analyzed the influence of patient variables on airborne SARS-CoV-2 genome detection. Methods: Sixty-nine COVID-19 patients...
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153
A FIASCO-Based Approach for Detection and Diagnosis of Puccinia graminis f. sp. tritici in China
Wydane 2014-11-01Hasła przedmiotowe: Dokumenty pełnotekstoweStem or black rust of wheat, caused by the fungus Puccinia graminis Pers. f. sp. tritici Eriks. & E. Henn. (Pgt), has historically caused severe losses to wheat (Triticum aestivum L.) production worldwide. In the Fujian and Guangdong provinces of China, six moderate-to-severe epidemics of wh...
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154
Detecting DNA of multispecies dinoflagellate cysts in the sediment from three estuaries of Makassar strait and fishing port using CO1 primer: Is it CO1 primer suitable for detectin...
Wydane 2023-10-01“…The conventional method for identifying dinoflagellate cyst in natural sediment requires morphological observation, isolating, germinating and cultivating the cysts. PCR is a highly sensitive method for detecting dinoflagellate cyst in the sediment. …”Most dinoflagellate had a resting cyst in their life cycle. This cyst was developed in unfavorable environmental condition. The conventional method for identifying dinoflagellate cyst in natural sediment requires morphological observation, isolating, germinating and cultivating the cysts. PCR is...
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155
Development of an improved RT-qPCR Assay for detection of Japanese encephalitis virus (JEV) RNA including a systematic review and comprehensive comparison with published methods.
Wydane 2018-01-01“…<h4>Background</h4>Japanese encephalitis virus (JEV) is a major cause of encephalitis in Asia, and the commonest cause of mosquito-borne encephalitis worldwide. Detection of JEV RNA remains challenging due to the characteristic brief and low viraemia, with 0-25% of patients positive, and the mainstay of diagnosis remains detection of anti-JEV IgM antibody.…”<h4>Background</h4>Japanese encephalitis virus (JEV) is a major cause of encephalitis in Asia, and the commonest cause of mosquito-borne encephalitis worldwide. Detection of JEV RNA remains challenging due to the characteristic brief and low viraemia, with 0-25% of patients positive, and...
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156
ANTIMICROBIAL RESISTANCE PROFILING OF SELECTED E. coli ISOLATES AND DETECTION OF ESBL/PAMPC-ENCODING GENES IN BROILER FLOCKS IN BOSNIA AND HERZEGOVINA USING REAL-TIME PCR
Wydane 2025-07-01“…Additionally, a multiplex real-time PCR assay was used to detect extended-spectrum β-lactamase (ESBL)- and carbapenemase-encoding genes. …”Antimicrobial resistance (AMR) is a growing global issue, driven by the nontargeted use of antimicrobials in livestock. Poultry, particularly broilers, may serve as significant reservoirs for resistant Escherichia (E.) coli strains. This study aimed to isolate E. coli from broiler flocks and evalua...
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157
Analytical performance evaluation of a multiplex real-time RT-PCR kit for simultaneous detection of SARS-CoV-2, influenza A/B, and RSV
Wydane 2025-07-01“…This cross-sectional study, conducted from September to November 2023, evaluated the analytic performance of the LabTurbo multiplex real-time reverse transcription polymerase chain reaction (RT-PCR) kit for the simultaneous detection of these viruses. …”Differentiating between influenza, respiratory syncytial virus (RSV), and SARS-CoV-2 based on clinical symptoms alone can be challenging due to their overlap. This cross-sectional study, conducted from September to November 2023, evaluated the analytic performance of the LabTurbo multiplex real-time...
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158
Detection of Clarithromycin Resistance in Helicobacter pylori Using the AllplexTM H. pylori & ClariR Assay and the Ezplex® HP-CLA Real-Time PCR Kit
Wydane 2025-03-01“…This study evaluated the diagnostic performance of two frequently used polymerase chain reaction (PCR) methods (AllplexTM H. pylori & ClariR Assay [Seegene] and Ezplex® HP-CLA Real-time PCR [SML Genetree]) to detect H. pylori infection and identify point mutations associated with clarithromycin resistance. …”Objectives The success of Helicobacter pylori eradication using clarithromycin-based triple therapy relies on the bacteria being sensitive to clarithromycin. This study evaluated the diagnostic performance of two frequently used polymerase chain reaction (PCR) methods (AllplexTM H. pylori & Clar...
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159
Development and Testing of the Method for the Detection of Lassa virus RNA, Based on real-Time Polymerase Chain reaction with reverse Transcription
Wydane 2018-12-01“…Objective of the study was the development of a method for the detection and quantitative analysis (realtime RT-PCR) to identify genetic markers of Lassa virus - LASV-Fl. …”Abstract. Objective of the study was the development of a method for the detection and quantitative analysis (realtime RT-PCR) to identify genetic markers of Lassa virus - LASV-Fl. Materials and methods. We utilized all the available in the GenBank database (https://www.ncbi.nlm.nih.gov/genbank/) La...
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160
Droplet Digital PCR or Real-Time PCR as a Method for Quantifying SARS-CoV-2 RNA in Plasma—Is There a Difference?
Wydane 2025-05-01“…The aim of this study is to ascertain whether qRT-PCR (reverse transcriptase real-time PCR) or RT-ddPCR (reverse transcriptase digital droplet PCR) is more effective for detecting SARS-CoV-2 RNA (severe acute respiratory syndrome coronavirus 2 RNA) in blood plasma from COVID-19 (coronavirus infectious disease-19) patients. …”The aim of this study is to ascertain whether qRT-PCR (reverse transcriptase real-time PCR) or RT-ddPCR (reverse transcriptase digital droplet PCR) is more effective for detecting SARS-CoV-2 RNA (severe acute respiratory syndrome coronavirus 2 RNA) in blood plasma from COVID-19 (coronavirus infectio...
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