Rezultaty - PCR detection
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121
Development of a Quadruplex RT-qPCR Assay for Rapid Detection and Differentiation of PRRSV-2 and Its Predominant Genetic Sublineages in China
Wydane 2025-06-01“…Positive samples selected for sequencing were further confirmed using ORF5-based phylogenetic analysis and NSP2 deletion pattern comparison, which aligned with RT-qPCR detection results. Field application primarily detected NADC30-like PRRSV, while further validation is still needed for HP-like and NADC34-like strains. …”Background: Porcine Reproductive and Respiratory Syndrome (PRRS) is a highly contagious disease characterized by reproductive failure in sows and severe respiratory disorders across all swine ages, causing significant economic losses. In China, the PRRSV epidemiological landscape is complex, with th...
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122
Performance of DiaRD-HPV Rt-PCR Kit for Detection of High-Risk Human Papilloma Virus Genotypes: A Preliminary Study
Wydane 2025-06-01“…DNA was isolated from cervical specimens in PreservCyt transport medium using the Diarex Viral DNA/RNA extraction kit. Multiplex PCR was performed following the DiaRD-HPV Rt-PCR kit protocol. …”Objectives: Rapid detection and identification of high-risk human papillomavirus (hrHPV) genotypes in cervical specimens has a crucial importance for management of patients. In this study, we evaluated the performance of the novel DiaRD-HPV Rt-PCR kit developed for screening HPV in cervical samples...
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123
Development of a Reagent Kit for the Quantitative Determination of Hepatitis B Virus (HBV) DNA in Clinical Material by PCR with Hybridization-Fluorescence Detection
Wydane 2023-09-01“…The polymerase chain reaction (PCR) is currently the most sensitive method for the detection and quantification of HBV. …”Relevance. Hepatitis B virus (HBV) is one of the most common viral infections affecting people worldwide and can lead to chronic hepatitis, cirrhosis and hepatocellular carcinoma. Currently 3% of the world's population are infected with hepatitis B virus and are at risk of developing life-threa...
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124
Multiplex PCR for Detection of Exfoliative Toxins, and Toxic Shock Syndrome Toxin 1 Genes in MRSA Strains of Clinical Staphylococcus aureus Isolates
Wydane 2023-02-01“… This research approaches to investigate toxin gene content of the methicillin resistant Staphylococcus aureus strains that are clinically collected from human skin infection cases by performing multiplex polymerase chain reaction (PCR) technique (irrespective of whether the strain produces the toxin or not) and for that a single PCR reaction was employed, two pairs of exfoliative toxin and one pair toxic shock syndrome toxin-1. …”This research approaches to investigate toxin gene content of the methicillin resistant Staphylococcus aureus strains that are clinically collected from human skin infection cases by performing multiplex polymerase chain reaction (PCR) technique (irrespective of whether the strain produces the toxi...
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125
A novel real-time RT-PCR with TaqMan-MGB probes and its application in detecting BVDV infections in dairy farms
Wydane 2015-08-01“…A real-time RT-PCR assay using TaqMan-MGB probes was developed to detect and type the bovine viral diarrhea virus (BVDV) in cattle. …”A real-time RT-PCR assay using TaqMan-MGB probes was developed to detect and type the bovine viral diarrhea virus (BVDV) in cattle. Universal primers and TaqMan-MGB probes were designed from the 5′-untranslated region of known pestiviral sequences. Prior to optimizing the assay, cRNAs were transcrib...
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126
Novel TaqMan® real-time PCR targeting invJ gene for 8-h detection of Salmonella from food matrices
Wydane 2025-07-01“…In this study, a faster and sensitive TaqMan® real-time PCR assay was developed with a 6 h enrichment paradigm for the direct detection of Salmonella from food matrices using a novel target gene, invJ, which is part of the Salmonella Type 3 Secretion System (T3SS). …”Salmonella, a well-known food-borne zoonotic pathogen, is the causative agent of Salmonellosis affecting public health in both developed and developing countries. Traditional Salmonella detection methods are time-consuming, involving multiple steps like pre-enrichment, selective plating, and biochem...
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127
Detection of Sak Gene and Expression of Staphylokinase in Different Clinical Isolates of Staphyloccocus spp.
Wydane 2018-04-01Dokumenty pełnotekstoweThis study had investigated the presence of Staphylokinase (SAK) phenotypically and genetically. A total of 100 Staphylococcus spp. samples were isolated from different clinical cases. Morphological, biochemical tests and for further conformation; Viteck2 technique were adopted to isolate and diagn...
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Identification of orthohantaviruses detected for the first time in the Republic of Belarus
Wydane 2025-03-01“…Screening of 613 samples from small mammals caught in the territory of the Republic of Belarus was carried out by the real time PCR method using the test system «Belar-GLPS-PCR/RV». …”Introduction. Monitoring of hemorrhagic fever with renal syndrome (HFRS) pathogens in the Republic of Belarus is necessary and relevant, since the number of HFRS cases in the population has increased in recent years, and genetic characteristics of the pathogens remain unidentified. Aim of the stu...
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129
Complex Approach to Species-Specific Detection of Cowpox Virus
Wydane 2016-12-01“…Implemented was complex approach to species-specific detection of CPV using multiplex real-time PCR variant for simultaneous multilocus detection on the basis of three independent target genes of CPV, to genus-specific detection for the exclusion of false-negative results, and additional oligonucleotide primers and probes, providing for specific detection of EV, aimed at the exclusion of false-positive results.…”Objective of the study is to develop and validate the method for species-specific detection of cowpox virus (CPV). Materials and methods. Utilized were oligonucleotide primers and fluorescence-labeled probes for genus-specific detection of orthopoxviruses (OPV) and species-specific detection of cowp...
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130
Detection of CTX-M-type ESBLs from Escherichia coli Clinical Isolates from a Tertiary Hospital, Malaysia
Wydane 2019-09-01“…The present study aims to detect CTX-M-type ESBL from Escherichia coli clinical isolates and to analyze their antibotic susceptibility patterns. …”The present study aims to detect CTX-M-type ESBL from Escherichia coli clinical isolates and to analyze their antibotic susceptibility patterns. One hundred of E. coli isolates were collected from different clinical samples from a tertiary hospital. ESBL positivity was determined by the disk diffusi...
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131
Molecular Detection of Cryptosporidium parvum by Specific Gene (Heat Shock Protein) in Children at Babylon Center
Wydane 2025-04-01Dokumenty pełnotekstoweBackground: Cryptosporidium is a diarrhea-causing single-celled parasite that infects the gastrointestinal tract of humans, livestock, birds, and wildlife populations. Objectives: Molecular detection of C. parvum by specific genes in children. Materials and Methods: A total of 300 stool samples were...
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132
Detection and characterization of Hepatitis E virus from commercial rabbit livers in Hebei, China
Wydane 2021-12-01Dokumenty pełnotekstoweRabbit hepatitis E virus (HEV) has been reported for years and is thought to have the potential for zoonotic transmission from rabbits to humans. As reported, HEV genotype 3 (gt3) is the most prevalent form of HEV in rabbits. To determine the prevalence of HEV in commercial rabbit livers, 176 liver...
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133
Evaluation of RT-LAMP for SARS-CoV-2 Detection in Animal Feces
Wydane 2025-05-01“…We validated a commercial, pH-based, colorimetric RT-LAMP assay for the detection of SARS-CoV-2 RNA in animal feces. The comparator assay was rRT-PCR. …”The wide host range, potential lethality, and zoonotic potential of SARS-CoV-2 infection in animals highlights the need for additional surveillance strategies. We validated a commercial, pH-based, colorimetric RT-LAMP assay for the detection of SARS-CoV-2 RNA in animal feces. The comparator assay wa...
Dokumenty pełnotekstowe
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134
Enhancing Candida auris diagnosis: a comprehensive evaluation of VIASURE Candida auris real-time PCR detection kit for rapid diagnostic responses
Wydane 2025-07-01“…This study aims to evaluate the VIASURE Candida auris Real-Time PCR Detection Kit (Certest Biotec) for diagnostic performance and analytical specificity compared to the standard methodology based on culture and MALDI-TOF MS characterization. …”ABSTRACT Candida auris, a yeast that is highly resistant to antifungal treatments, represents a significant healthcare concern due to its rapid spread through surface contamination and patient colonization. A recent outbreak at the Miguel Servet University Hospital in Zaragoza, Spain, highlighted th...
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135
Detection of residual <i>E. coli</i> and CHO<i></i>host-cell<i></i>DNA in recombinant proteins by qPCR
Wydane 2018-02-01“…The report on successful adaptation of the methods of residual E. coli and CHO DNA detection by qPCR using fluorescent probes was provided. …”Production of recombinant proteins is a steadily growing industry in Russia and all over the world. Both producer and substance should be properly characterized and tested against all safety criteria. One of the mandatory requirements to the mentioned drugs is the assessment of the content of residu...
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136
Simultaneous detection and differentiation of Mycobacterium tuberculosis and nontuberculous mycobacteria in smear-negative sputum by a multiplex PCR assay: a clinical feasibility study
Wydane 2025-07-01Hasła przedmiotowe: Dokumenty pełnotekstoweABSTRACT Accurate and rapid differentiation of Mycobacterium tuberculosis (M. tuberculosis) and nontuberculous mycobacteria (NTM) remains challenging, especially in smear-negative samples. Routine diagnostic methods often lack specificity and rapidity, highlighting the urgency of developing applicat...
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137
The quadruplex fluorescent quantitative PCR method for the simultaneous detection of respiratory diseases in quail: Pasteurella multocida, Avibacterium paragallinarum, Mycoplasma gallisepticum, and Mycoplasma synoviae
Wydane 2025-06-01“…These results were consistent with those obtained using previously established methods.DiscussionA highly sensitive, specific, rapid, and efficient quadruplex fluorescence quantitative PCR assay was successfully developed for the simultaneous detection and identification of Pasteurella multocida, Avibacterium paragallinarum, Mycoplasma gallisepticum, and Mycoplasma synoviae.…”BackgroundThe quail farming industry constitutes an important component of China’s agricultural sector. However, it is frequently threatened by various bacterial and mycoplasmal infections, particularly respiratory diseases caused by Pasteurella multocida, Avibacterium paragallinarum, Mycoplasma gal...
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138
A SINGLE – CENTER STUDY ON THE INCIDENCE OF PCR–DETECTED INFLUENZA A/B RNA IN PATIENTS WITH SEVERE ACUTE RESPIRATORY DISEASE (2015-2019)
Wydane 2025-06-01“…Influenza A and B virus RNA was detected using real-time PCR. Results. During the observation period, 49% of patients tested positive for influenza. …”Influenza virus infection remains the leading cause of morbidity and mortality in the autumn-winter period. Along with insights into circulating virus subtypes and seasonal trends, reliable data on the prevalence of influenza among patients with acute respiratory illnesses is essential for guiding t...
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139
Development of a technique for DNA detection and identification of toxigenic strains of Clostridium botulinum types A, B, E by the Real-Time PCR method
Wydane 2023-07-01Hasła przedmiotowe: Dokumenty pełnotekstoweBotulism is dangerous toxic infection caused by a toxin produced by the bacterium Clostridium botulinum. The mortality rate from botulism can reach 70% of all cases of illness in case of untimely initiation of treatment. The pathogenesis of botulism involves the damage to the central nervous system...
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Improvement of Diagnostic System in a Real-Time RT-PCR “AmpliSens EBOV (ZAIRE)-FL” Format for Zaire ebolavirus RNA Detection
Wydane 2015-09-01Dokumenty pełnotekstoweTaking into consideration experience in utilization of “AmpliSens EBOV (ZAIRE)-FL” in the setting of SAET mobile laboratory complex, carried out has been the improvement of the panel with a view to elapsed time reduction and analytical sensitivity enhancement. It provides for the analytical sensitiv...
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