Rezultaty - PCR detection
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101
Validation of a Real-Time PCR Assay for Fully Automated Detection of <i>Bacillus cereus</i> in Donor Human Milk
Wydane 2025-07-01“…In this study we developed and validated a non-commercial real-time PCR assay for the detection of <i>Bacillus cereus</i> (BC test) in DHM specimens on a fully automated high-throughput platform, the cobas<sup>®</sup> 6800 system. …”Donor human milk (DHM) can harbor microbial contaminants that cause serious infections in premature infants. <i>Bacillus cereus</i> is a pathogen frequently found in DHM, capable of forming spores that can resist Holder pasteurization (62.5 °C, 30 min). Since no microbial growth is accep...
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102
Droplet Digital PCR for the Detection of <i>Pseudomonas savastanoi</i> pv. <i>savastanoi</i> in Asymptomatic Olive Plant Material
Wydane 2025-06-01“…The PELLET from the spiked sample preparation achieved a limit of detection of 10 CFU/mL for both molecular tests. The ddPCR, combined with the PELLET preparation, offers a highly sensitive and reliable tool for detecting <i>P. savastanoi</i> pv. …”Olive knot disease, caused by <i>Pseudomonas savastanoi</i> pv. <i>savastanoi</i>, severely impacts olive tree yield and oil quality. Early and accurate detection of the bacterium’s presence, particularly in asymptomatic plants, is crucial for effective disease management. Th...
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103
Detection of Lineage IV Peste Des Petits Ruminants Virus by RT-qPCR Assay via Targeting the Hemagglutinin Gene
Wydane 2025-07-01“…The results indicated that this method could detect approximately six copies of PPRV RNA, indicating high sensitivity. …”Peste des petits ruminants virus (PPRV) has been classified into four lineages based on the nucleocapsid and fusion genes, with lineage IV strains being the most widely distributed. In Africa, recent epidemiological data revealed that PPRV lineage IV is increasingly displacing other lineages in prev...
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104
Detection of Toxoplasma gondii and High-Risk Human Papillomaviruses in FFPE Malignant and Benign Breast Lesions Using Real-Time PCR
Wydane 2025-06-01“…According to the Modified Bloom-Richardson classification, 55.15% of malignant breast tumor samples were grade 2, 32.4% were grade 3, and 12.5% were grade 1. Real-time PCR analysis did not detect T. gondii DNA or HPV DNA in any of these samples.Conclusion: Our findings do not support a role of T. gondii and HPV in breast cancer development. …”Selma Usluca,1,2 Ayfer Bakir,2 Ata Türker Arikok,3 Gizem Korkut,2 Gulsah Ceylan Yagiz,2 Murat Alper3 1Department of Medical Microbiology, Atilim University Medical School, Ankara, Türkiye; 2Department of Medical Microbiology, University of Health Sciences Ankara Etlik City Hospital, Ankara, Türkiye;...
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105
Performance Comparison of Droplet Digital PCR and Next‐Generation Sequencing for Circulating Tumor DNA Detection in Non‐Metastatic Rectal Cancer
Wydane 2025-05-01“…This study compared droplet digital PCR (ddPCR) and next‐generation sequencing (NGS) for ctDNA detection in localized rectal cancer before and after surgery. …”ABSTRACT Background and Objectives Circulating tumor DNA (ctDNA) can potentially identify rectal cancer patients benefiting from neoadjuvant and adjuvant therapy. This study compared droplet digital PCR (ddPCR) and next‐generation sequencing (NGS) for ctDNA detection in localized rectal cancer befor...
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106
Tracking adipose-derived mesenchymal stromal cells in the eye: Integrating IVIS imaging and Alu PCR for enhanced detection of human cells
Wydane 2025-12-01“…Genetically modified cells express luciferase, allowing for the detection of light emission when luciferin is administered. …”Introduction: Cell transplantation finds broad applications in medical science, with applications ranging from stem cell therapies to cancer research. Despite its widespread use, inherent risks such as tumor formation and immune rejection necessitate a comprehensive understanding of transplanted cel...
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107
Droplet Digital PCR Provides Highly Sensitive and Accurate Opsin Gene SNP Detection From Wild Primate Fecal Samples
Wydane 2025-02-01Dokumenty pełnotekstoweABSTRACT Evaluating field‐sourced samples with poor‐quality and low‐quantity DNA, like animal feces, presents significant challenges in the field of molecular biology. Nonetheless, recent innovations in PCR technology are promoted as effective tools to overcome many of these issues. Here, we evaluat...
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108
Establishment of a TaqMan qPCR method with MGB probe for the specific detection of BVDV field strains circulating in China
Wydane 2025-07-01“…In this study, a TaqMan real-time quantitative PCR (qPCR) assay utilizing a minor groove binder (MGB) probe was developed for the detection of BVDV, with a focus on strains currently circulating in China. …”Bovine viral diarrhea virus (BVDV), a highly mutable pathogen, poses a significant threat to the cattle industry in China. Therefore, the development of a rapid, sensitive, and specific diagnostic assay is essential for effective surveillance and control. In this study, a TaqMan real-time quantitati...
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109
Specificities of multi-primer polymerase chain reaction optimization for the detection of infectious pneumonia agents in human
Wydane 2021-07-01“…The objectives of this work are the development of a multi-primer system based on the polymerase chain reaction (PCR) aimed at the simultaneous detection of six bacterial pathogens that cause human pneumonia and the determination of the parameters important for the optimization of this multi-primer system, including solid-phase PCR systems (biological microarrays).Methods. …”Objectives. The objectives of this work are the development of a multi-primer system based on the polymerase chain reaction (PCR) aimed at the simultaneous detection of six bacterial pathogens that cause human pneumonia and the determination of the parameters important for the optimization of this m...
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110
CLINICAL INFORMATIVE VALUE IN THE DIAGNOSTIC TESTING FOR DETECTION OF ROTAVIRUS INFECTIONS IN ADULT PATIENTS WITH ACUTE DIARRHEA
Wydane 2018-12-01“…Specific examination for the detection of various pathogens of AD along with standard cultural, serologic (based on the determination of specific antibodies) and ELISA methods included the study of the patient’s feces by PCR. …”The paper suggests the results of retrospective cohort study. We analyzed the results of clinical and laboratory examination of 102 hospitalized adult patients with acute diarrhea (AD) of moderate severity and positive feces test for rotavirus. Specific examination for the detection of various patho...
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111
Development and Application of Species-Specific qPCR and RPA Assays for the Lettuce Wilt Pathogen, Globisporangium uncinulatum (syn. Pythium uncinulatum)
Wydane 2025-06-01“…The RPA assay was able to detect down to 5,000 fg of G. uncinulatum DNA from a crude plant extract. …”Pythium wilt of lettuce (Lactuca sativa L.), caused by Globisporangium uncinulatum (syn. Pythium uncinulatum), has become an important disease of lettuce in the Salinas Valley, California, since its identification in 2011. Losses attributed to G. uncinulatum in 2022 were estimated between 47 and 77...
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112
Using the molecular methods in detection of antibiotics resistance genes in bacteria of Typhoi (Salmonella typhi)
Wydane 2014-06-01Dokumenty pełnotekstoweThis study was carried out for direct detection of typhi and some of its multidrug resistance genes(tem,capt,gyrA&sul2)which encode for resistance to (Ampicillin, Chloramphenicol,Ciprofioxacin,Co-trimoxazole)by using Polymerase Chain Reaction technique .(71)blood samples for people suffering f...
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113
DETECTION OF CERTAIN GENETIC MARKERS OF THE PATHOGENIC FACTORS IN AUTOSTRAINS KLEBSIELLA SPP. IN INFANTS
Wydane 2014-04-01Dokumenty pełnotekstoweThe article presents the results of detection of genetic determinants of pathogenicity in 44 strains of Klebsiella (Klebsiella pneumoniae, Klebsiella oxytoca), isolated from the intestinal habitat of infants with intestinal dysbiosis. The presence of the uge gene was detected in 92 % of cases in K....
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114
Establishment and Application of MCRV Detection Method for Larvae of Mud Crab, Scylla paramamosain
Wydane 2025-08-01Hasła przedmiotowe: Dokumenty pełnotekstoweMud crab reovirus (MCRV) present in Scylla paramamosain larvae poses notable risks to the late stage of mud crab aquaculture. The cultivation of MCRV-free larvae and prevention of virus transmission at its source have become critical issues that must be urgently addressed in mud crab aquaculture. Ow...
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115
Molecular Detection and characterization of viruses infecting greenhouse-grown tomatoes in Albania
Wydane 2025-05-01Dokumenty pełnotekstoweDuring the 2023–2024 growing season, a total of 45 tomato greenhouses were visited across five major production regions of Albania (Berat, Lushnje, Fier, Tirana, and Shkodra). A total of 196 greenhouse-grown tomato leaf samples, representing 31 varieties, were collected from plants showing virus-lik...
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116
Advanced Laboratory Methods for Detecting Yellow Fever Pathogen
Wydane 2021-07-01Dokumenty pełnotekstoweYellow fever is an acute infectious disease of viral nature, the causative agent of which is vector-borne –is transmitted through the bites of infected mosquitoes. Massive epidemics caused by the yellow fever virus are observed in the countries of Africa, South and Central America annually. Imported...
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117
The Effectiveness of PCR in Diagnosis of Fungal Keratitis
Wydane 2020-12-01“…To evaluate the effectiveness of polymerase chain reaction (PCR) for the detection of fungal etiology in comparison with the conventional diagnostic methods in cases with suspected fungal corneal ulcer.Patients and methods. …”Fungi implicated in mycotic keratitis include different species. Conventional methods for the diagnosis of fungal keratitis include staining of corneal scarpings, culture medium (Sabouraud agar) for isolating fungi.Purpose. To evaluate the effectiveness of polymerase chain reaction (PCR) for the det...
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118
Development of a TaqMan One-Step Quantitative PCR Assay for the Simultaneous Detection of Novel Goose Parvovirus and Novel Duck Reovirus
Wydane 2025-07-01Hasła przedmiotowe: Dokumenty pełnotekstoweThe novel goose parvovirus (NGPV) and the novel duck reovirus (NDRV) are pathogens that can substantially affect the growth and development of ducklings, causing considerable economic losses to duck farms. Therefore, a timely, rapid, accurate, and high-throughput diagnosis and identification of vira...
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119
Detection of SARS‑CoV‑2 S Gene Mutations Using PCR during Seasons of Increased Incidence of Coronavirus Infection in the Chuvash Republic
Wydane 2024-01-01“…Under conditions of the clinical diagnostic laboratory of the Federal Center for Traumatology, Orthopedics and Endoprosthetics of the Ministry of Health of Russia (Cheboksary), samples, containing SARS‑CoV‑2 RNA, taken in January-February and July-October, 2022 were tested using reverse transcription PCR. The “MBS-Test SARS‑CoV‑2 RNA” (Technical Specifications 21.20.23-068-26329720-2021, Russia) and “AmpliTest SARS‑CoV‑2 VOC v.3” (Series V017, Certificate of Registration No. 2022/16307, Russia) were utilized in compliance with the manufacturer’s instructions.Results and discussion. …”Mutations in the SARS‑CoV‑2 genome make it possible to effectively escape defense mechanisms of the host, which explains the spread of infection among vaccinated or previously affected by the virus individuals.The aim of the study was to investigate the dynamics of mutations in the SARS‑CoV‑2 virus...
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Multiplex PCR Technique for Simultaneous Detection and genotyping of E. granulosus, E. multilocularis, and other Taeniidae in some intermediate hosts in Erbil Province
Wydane 2024-04-01Dokumenty pełnotekstoweOne of the most significant helminthozoonosis in Iraq and around the world is cystic echinococcosis, which occur by the etiologic agent of Echinococcus granulosus and it’s distinguished by considerable intra-specific variability (genotypes G1–G10). DNA was extracted from 48 isolated cysts (25 sheep...
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