Rezultaty - PCR detection
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81
Development of a TaqMan-based quantitative real-time PCR for the detection of Porcine circovirus type 3
Wydane 2025-07-01Hasła przedmiotowe: Dokumenty pełnotekstowePorcine circovirus type 3 (PCV3) is a recently identified member of the porcine circovirus family, primarily associated with conditions such as dermatitis and nephropathy syndrome, reproductive failure, and multisystem inflammatory lesions in swine. There has been a significant increase in the preva...
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82
Species-specific PCR-based assays for identification and detection of Botryosphaeriaceae species causing stem blight on blueberry in China
Wydane 2016-03-01“…This PCR protocol could detect as low as 100 pg to 1 ng of purified fungal DNA. …”Botryosphaeriaceae species are important causal agents of blueberry stem blight worldwide. Blueberry stem blight has become an important disease, potentially affecting the quality and production of blueberries in China. It is difficult and time-consuming to identify at the species level using morpho...
Dokumenty pełnotekstowe
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83
Droplet digital RT-PCR method for SARS-CoV-2 variants detection in clinical and wastewater samples
Wydane 2025-07-01“…ObjectiveTo establish a sensitive, specific, and precise quantitative detection method for SARS-CoV-2 variants using droplet digital RT-PCR (RT-ddPCR).MethodsDual primer-probe sets targeting the SARS-CoV-2 nucleocapsid (N) and spike (S) genes were designed. …”ObjectiveTo establish a sensitive, specific, and precise quantitative detection method for SARS-CoV-2 variants using droplet digital RT-PCR (RT-ddPCR).MethodsDual primer-probe sets targeting the SARS-CoV-2 nucleocapsid (N) and spike (S) genes were designed. The annealing temperature for RT-ddPCR was...
Dokumenty pełnotekstowe
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84
Comprehensive Virome Profiling of Apple Mosaic Disease-Affected Trees in Iran Using RT-PCR and Next-Generation Sequencing
Wydane 2025-07-01“…NGS further revealed three additional pathogens: citrus concave gum-associated virus (CCGaV), apple hammerhead viroid (AHVd), and apricot vein clearing-associated virus (AVCaV), which were subsequently detected across the collection by RT-PCR. AGCaV was most prevalent (47.6%), followed by ACLSV (45.8%), HSVd (27.6%), AVCaV (20.5%), ASGV (17%), AHVd (15.2%), ASPV (14.1%), CCGaV (4.7%), and ApMV (3.5%). …”Apples (<i>Malus domestica</i>), one of Iran’s oldest cultivated fruit crops, hold considerable economic importance. In this study, 170 apple leaf samples representing various commercial cultivars were collected across the country. RT-PCR screening targeted five common apple-infecting vi...
Dokumenty pełnotekstowe
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85
Molecular detection of ompX gene related to immune resistance in Salmonella typhi
Wydane 2025-08-01“…This work aims to isolate and identify S.enterica through PCR and detect the ompx gene in clinical samples from human subjects. …”Background: Salmonella is known for its ability to invade and survive within host cells, which is attributed to various virulence factors it possesses. These virulence determinants enable the bacteria to adhere to and invade host cells, bypass host immune defenses, and replicate within host cells, l...
Dokumenty pełnotekstowe
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86
Picobirnaviruses: prevalence, genetic diversity, detection methods
Wydane 2020-10-01“…Two strategies of RT-PCR as main PBV detection methods are considered. …”This article presents a general overview of the prevalence, genetic diversity and detection methods of picobirnaviruses (PBVs), which are small, non-enveloped icosahedral viruses with a segmented double-stranded RNA genome consisting of two segments taxonomically related to the genus Picobirnavirus...
Dokumenty pełnotekstowe
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87
Detection of Apple Chlorotic Leaf Spot Virus in Tissues of Pear Using In Situ RT-PCR and Primed In Situ Labeling
Wydane 2012Dokumenty pełnotekstoweNone
Elektroniczne Rozdział
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88
A phosphoprotein gene RT-qPCR assay for detecting all lineages of peste des petits ruminants virus
Wydane 2025-07-01“…The assay was assessed for its sensitivity, specificity, and repeatability in the detection of field samples and was compared with the standard detection method. …”Peste des petits ruminants (PPR) is a highly contagious disease caused by peste des petits ruminants virus (PPRV). PPRV is classified into four lineages based on the nucleocapsid (N) or fusion (F) genes. We established a TaqMan quantitative real-time reverse transcription polymerase chain reaction (...
Dokumenty pełnotekstowe
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89
A duplex RT-PCR assay for detection of H9 subtype avian influenza viruses and infectious bronchitis viruses
Wydane 2016-09-01“…Therefore, developing a sensitive and specific method is of importance to simultaneously detect and differentiate H9 AIV and IBV. In this study, a duplex RT-PCR (dRT-PCR) was established. …”H9 subtype avian influenza virus (AIV) and infectious bronchitis virus (IBV) are major pathogens circulating in poultry and have resulted in great economic losses due to respiratory disease and reduced egg production. As similar symptoms are elicited by the two pathogens, it is difficult for their d...
Dokumenty pełnotekstowe
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90
Comparison of fragment analysis and PCR electrophoresis methods for the detection of FLT3‑ITD mutations in patients with acute myeloid leukemia
Wydane 2022-11-01“…The first, more accessible and cheaper method is the method of pCR electrophoresis and the second, more expensive and requiring special equipment, is the fragment analysis method, which allows not only to detect a mutation and determine the repeat length, but also to quantify or calculate AR.Aim. …”Background. The presence of the FLT3-ITD mutations in patients with AML serves as a marker of poor prognosis, which is included in the ELN 2017 risk stratification guideline. The main criterion for dividing patients into groups according to the predicted outcomes was the allelic ratio (AR) with a cu...
Dokumenty pełnotekstowe
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91
Diagnostic utility of real-time RT-PCR for chikungunya virus detection in the acute phase of infection: a retrospective study
Wydane 2025-12-01“…Samples were subjected to nucleic acid extraction followed by chikungunya real-time RT-PCR using a standardized in-house protocol. The samples that tested positive for Chikungunya by real-time RT-PCR were further tested for detecting anti-Chikungunya IgM antibodies using enzyme-linked immunosorbent assay. …”Background Chikungunya fever is a viral disease spread by Aedes mosquitoes, reported in over 110 countries. In India, it was first detected in Kolkata in 1963 and is now widespread. Diagnosis often relies on detecting anti-chikungunya IgM antibodies, but these may not be present during the acute pha...
Dokumenty pełnotekstowe
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92
Detection of Unculturable Fungi in Soil Regions of Aziz Awa in Sulaimani province
Wydane 2023-01-01Dokumenty pełnotekstoweUnculturable and non-sporulting fungi represent a great challenge in studying biotrophic, endotrophic and mycorrhizal fungal groups. In this study collection of soil sample from region Aziz Awa in sulaimani province and using culture-dependent and culture in-dependent techniques for cultivation of...
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93
Molecular detection of some Staphylococcal enterotoxins in the meat of slaughtered animals in abattoirs, Mosul- Iraq
Wydane 2025-07-01“…For the purpose of further analysis to detect the enterotoxin genes, the isolates that were previously confirmed as S. aureus by using specific Nuc gene, PCR was applied to 46 random isolates to identify S. enterotoxins (sea, seb, sec, sed, sef, and Tsst genes) which gave a positive result for Sea, seb, sec, and Tsst genes with molecular weight 219, 478, 257 and 559 bp, respectively, except the seD and seE gene are not detected in this study, moreover some isolates carrying either one or more than one of enterotoxins genes. …”Foodborne pathogens are an international health problem, especially Staphylococcus (S.) aureus, which is considered one of the most important foodborne pathogens. The study aimed to isolate and identify the S. aureus bacteria from the meat of slaughtered animals in Mosul abattoirs during the period...
Dokumenty pełnotekstowe
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94
Lysis Buffers for Detecting Pork Content in Food Products
Wydane 2025-07-01“…This issue has prompted the development of halal authentication techniques, including deoxyribonucleic acid (DNA) isolation followed by polymerase chain reaction (PCR) amplification to detect pork DNA. The main purpose of this research was to modify the lysis buffer using tris-ethylenediaminetetraacetic acid, cetyl trimethylammonium bromide, salt, alkaline, and urea. …”The increasing number of counterfeit processed food products with non-halal ingredients, such as pork, has caused public concern about the halalness of Indonesian food products, especially in the Muslim community. This issue has prompted the development of halal authentication techniques, including...
Dokumenty pełnotekstowe
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95
CRISPR/Cas13a-Assisted accurate and portable hepatitis D virus RNA detection
Wydane 2023-12-01“…At present, there remains a lack of accurate and portable detection methods for HDV RNA. Here, we aim to establish a convenient, rapid, highly sensitive and specific method to detect HDV RNA using CRISPR–Cas13a technology.Methods We established fluorescence (F) and lateral flow strip (L) assays based on CRISPR–Cas13a combined with RT–PCR and RT-RAA for HDV RNA detection, respectively. we conducted a cohort study of 144 patients with HDV-IgG positive to evaluate the CRISPR–Cas13a diagnostic performance for identifying HDV in clinical samples, compared to RT–qPCR and RT-ddPCR.Results For synthetic HDV RNA plasmids, the sensitivity of RT–PCR-CRISPR-based fluorescence assays was 1 copy/μL, higher than that of RT–qPCR (10 copies/μL) and RT-ddPCR (10 copies/μL); for HDV RNA-positive samples, the sensitivity of RT-RAA-CRISPR-based fluorescence and lateral flow strip assays was 10 copies/μL, as low as that of RT–qPCR and RT-ddPCR, and the assay took only approximately 85 min. …”Background & Aims Hepatitis delta virus (HDV) infection accelerates the progression of chronic hepatitis B virus (HBV) infection, posing a large economic and health burden to patients. At present, there remains a lack of accurate and portable detection methods for HDV RNA. Here, we aim to establ...
Dokumenty pełnotekstowe
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96
Genetic Marker Detection of Natural-Focal Infectious Disease Pathogens in Samples of Ixodidae Ticks, Collected on the Territory of the Republic of Guinea
Wydane 2024-01-01“…Genetic markers of Crimean-Congo hemorrhagic fever and tick-borne encephalitis viruses, as well as Borrelia burgdorferi s.l., Ehrlichia chaffeensis, Ehrlichia muris, Anaplasma phagocytophilum, Coxiella burnetii, Rickettsia of the tick-borne spotted fever (TBSF) group, and Francisella tularensis were detected using polymerase chain reaction (PCR) and reverse transcription polymerase chain reaction (RT-PCR).Results and discussion. …”The circulation of a rather wide range of pathogens of natural-focal infectious diseases transmitted by ticks was detected in West Africa at different points of time: Borrelia, Rickettsia, Coxiella, Crimean-Congo hemorrhagic fever (CCHF), Bhanja, and bluetongue viruses, etc. Current epidemiological...
Dokumenty pełnotekstowe
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A sensitive SYBR Green RT-qPCR method for grapevine virus E and its application for virus detection in different grapevine sample types
Wydane 2020-07-01Hasła przedmiotowe: Dokumenty pełnotekstoweTo develop a rapid and high-sensitivity method for detection of grapevine virus E (GVE), a SYBR Green based real-time fluorescence quantitative RT-PCR method (RT-qPCR) was established. This method could be used to detect GVE specifically, and the sensitivity was about 100 times greater than conventi...
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SmartScreen‐AIS: A High‐Throughput qPCR Chip for Nationwide Surveillance of Aquatic Invasive Species
Wydane 2025-05-01Hasła przedmiotowe: “…early detection…”ABSTRACT Effective wildlife conservation requires frequent and widespread data on species occurrence. With the maturation of eDNA‐based monitoring—now widely recognized as sensitive, cost effective, and legally defensible—nationally coordinated eDNA strategies are beginning to take shape. Such ambit...
Dokumenty pełnotekstowe
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DETECTION OF TOXOPLASMOSIS AND SOME RISK FACTORS WITH THE INFECTION RATE IN LOCAL AND SHAMI GOATS
Wydane 2025-06-01Hasła przedmiotowe: “…Toxoplasmosis, nPCR, ELISA, risk factors, detection, infection…”This study was aimed to evaluate detection of T. gondii in blood DNA based on the B1 gene and serologically, also to investigate the effect of some risk factors (sex, ages, breeds and flocks). A total of 190 goats were included. The blood samples were obtained from the jugular vein and subjected to...
Dokumenty pełnotekstowe
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Environmental DNA Detects Endangered Texas Hornshell and Its Hosts
Wydane 2025-07-01“…Here, we developed novel quantitative PCR (qPCR) assays for the detection of the endangered Texas hornshell (Popenaias popeii) and two of its' host species, the gray redhorse (Moxostoma congestum) and blue sucker (Cycleptus elongatus), which are also species of greatest conservation need in New Mexico. …”ABSTRACT Environmental DNA techniques are continuously demonstrating their efficiencies over traditional survey methods for detecting rare species. Unionids have a need for focused conservation efforts as they represent some of the most imperiled aquatic species in North America. Freshwater mussels...
Dokumenty pełnotekstowe
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