Rezultaty - PCR detection
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Detecting the Helicobacter pylori 16S rRNA Gene in Dyspepsia Patients Using Real-Time PCR
Wydane 2019-05-01“…The coccoid form of H. pylori is difficult to detect either by culture or histopathology; however, it can be detected using molecular methods, such as real-time PCR. …”Background: early detection of H. pylori is essential to prevent the development of infections into gastric malignancies. The coccoid form of H. pylori is difficult to detect either by culture or histopathology; however, it can be detected using molecular methods, such as real-time PCR. The study wa...
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Development of a nested PCR assay for detecting Colletotrichum siamense and Colletotrichum fructicola on symptomless strawberry plants.
Wydane 2022-01-01“…Previous PCR-based methods developed for the diagnosis or detection of Colletotrichum acutatum and Colletotrichum gloeosporioides have used primers targeting the internal transcribed spacer region of ribosomal DNA, β-tubulin gene, or mating type gene. …”Anthracnose is a major disease of strawberry that seriously impacts the strawberry industry. To prevent the spread of anthracnose through symptomless plants, it is important to detect pathogenic Colletotrichum spp. at the latent infection stage in the nursery. Previous PCR-based methods developed fo...
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Nested PCR amplification of the mitochondrial hypervariable region for non-invasive eDNA detection of Cryptobranchus alleganiensis.
Wydane 2025-01-01“…Our nested PCR approach overcomes several shortcomings of previous eDNA detection methods for C. alleganiensis, most notably an order of magnitude improvement in the limit of detection for animal DNA in the field.…”Environmental DNA (eDNA) has proven an invaluable tool in detecting elusive, endangered, or otherwise hard-to-identify species in aquatic ecosystems. The Eastern hellbender salamander (Cryptobranchus alleganiensis) is a fully aquatic amphibian with an increasingly threatened population due to its re...
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Detection of Leishmania tropica Using Nested-PCR and Some of Their Virulence Factors in Thi-Qar Province, Iraq
Wydane 2021-03-01“…Conventional-PCR was performed for determination of some virulence factors (LPG1, GP63, CPA and PPG1 genes). …”Cutaneous leishmaniasis is one of endemic diseases in Iraq. It is considered as widely health problem and is an uncontrolled disease. The aim of the study is to identify of Leishmania species that cause skin lesions among patients in Thi-Qar Province, South of Iraq, also to detect some virulence fac...
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Biological and molecular characterization of tomato brown rugose fruit virus and development of quadruplex RT-PCR detection
Wydane 2021-07-01Hasła przedmiotowe: Dokumenty pełnotekstoweTomato brown rugose fruit virus (ToBRFV) is a novel tobamovirus firstly reported in 2015 and poses a severe threat to the tomato industry. So far, it has spread to 10 countries in America, Asia, and Europe. In 2019, ToBRFV was identified in Shandong Province (ToBRFV-SD), China. In this study, it was...
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Monitoring and detection of genetically modified soybean in animal feed using real-time PCR (2019-2024)
Wydane 2025-01-01“…A total of 94 samples were analyzed using sensitive molecular methods, including qualitative and quantitative PCR (Real-time PCR), in accordance with validated international protocols. …”The detection and monitoring of genetically modified organisms (GMOs) in animal feed is becoming increasingly important due to legal requirements related to labeling and control of authorization for placing GM products on the EU market. This study focuses on the presence and diversity of GM sequence...
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A Prevalence study of Entamoeba spp. in Basrah Province using Different Detection Methods
Wydane 2021-12-01Dokumenty pełnotekstoweThis study aims to determine the prevalence of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii by three methods of diagnosis (microscopic examination, cultivation and PCR) that were compared to obtain an accurate diagnosis of Entamoeba spp. during amoebiasis. Total (n=150) stool sa...
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Universally Primed PCR (UP-PCR) and its applications for taxonomy in Trichoderma
Wydane 2004-07-01“…This separates the strains into entities with high genetic similarity (DNA homology). UP-PCR has been used as an aid in taxonomy and species delineation, and to monitor biocontrol strains following their release into the environment by fingerprint characterisation of pure cultures and through direct detection in soil by amplification of UP-PCR-derived SCAR markers. …”Universally Primed PCR (UP-PCR) is a PCR fingerprinting method that has demonstrated its applicability in different aspects of mycology. These applications constitute analysis of genome structures, identification of species, analysis of population and species diversity, revealing of genetic relatedn...
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Possibilities of qPCR control of mycoplasma contamination of cell cultures
Wydane 2018-02-01“…The authors chose the most applicable qPCR method of all qPCR methods currently used for mycoplasma detection, and modified it in the following way: expensive MGB-probes which cannot be synthesized in Russia were substituted by ordinary fluorescence probes. …”Mycoplasmas are the main contaminants of cells cultures. They persist in cell cultures and can extensively affect host cell functions. Conducting experiments or producing protein in contaminated cultures are impractical. The aim of the study was to explore the possibility of quick detection of mycop...
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Detection of Rickettsial DNA in Ticksin Irkutsk Region
Wydane 2015-12-01“…Individually 470 Dermacentor nuttalli and 46 Haemaphysalis concinna ticks collected in Irkutsk Region were analyzed using RT-PCR test systems. Rickettsia contamination of D. nuttalli was 82,3% including 1,3% of the ticks with R. sibirica DNA. …”Individually 470 Dermacentor nuttalli and 46 Haemaphysalis concinna ticks collected in Irkutsk Region were analyzed using RT-PCR test systems. Rickettsia contamination of D. nuttalli was 82,3% including 1,3% of the ticks with R. sibirica DNA. H. concinna ticks were infected by rickettsia to 8,7% and...
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Results of Detecting SARS-CoV-2 and Other Pathogens of Community-Acquired Pneumonia by PCR in the Tyumen Region
Wydane 2020-10-01“…The aim of this work was to identify the causative agent of community-acquired pneumonia and coinfection using PCR study of biomaterial from patients.Materials and methods. …”The aim of this work was to identify the causative agent of community-acquired pneumonia and coinfection using PCR study of biomaterial from patients.Materials and methods. PCR testing of 268 samples from 258 patients was carried out to identify RNA/DNA of viral and bacterial pathogens of respirator...
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Development of high sensitive real-time PCR to detect mustard and other allergens of the family Brassicaceae in food samples
Wydane 2020-10-01“…As mustard is an allergen, it is necessary to control its presence. The development of PCR test-systems for its detection is complicated by the fact that this condiment can be made from seeds of various plant species (Brassica juncea, Brassica nigra, Sinapis alba) of the family Brassicaceae that are not closely related. …”Mustard is a commonly used condiment including in production of other food products. As mustard is an allergen, it is necessary to control its presence. The development of PCR test-systems for its detection is complicated by the fact that this condiment can be made from seeds of various plant specie...
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Multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis - a systemic review and meta-analysis.
Wydane 2013-01-01Dokumenty pełnotekstowe<h4>Background</h4>Blood culture is viewed as the golden standard for the diagnosis of sepsis but suffers from low sensitivity and long turnaround time. LightCycler SeptiFast (LC-SF) is a real-time multiplex polymerase chain reaction test able to detect 25 common pathogens responsible fo...
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Nested RT-PCR method for the detection of European avian-like H1 swine influenza A virus
Wydane 2016-05-01“…In this study, a nested reverse transcription (RT)-PCR assay was developed to detect EA H1 swine IAVs. …”Swine influenza A virus (swine IAV) circulates worldwide in pigs and poses a serious public health threat, as evidenced by the 2009 H1N1 influenza pandemic. Among multiple subtypes/lineages of swine influenza A viruses, European avian-like (EA) H1N1 swine IAV has been dominant since 2005 in China an...
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Detection of CADM1, MAL, and PAX1 Methylation by ddPCR for Triage of HPV-Positive Cervical Lesions
Wydane 2025-06-01“…<b>Methods:</b> Cervical samples from 73 HPV-positive women were analyzed using droplet digital PCR (ddPCR) to quantify methylation levels of <i>CADM1</i>, <i>MAL</i>, and <i>PAX1</i>. …”The aberrant DNA methylation of tumour suppressor genes, including <i>CADM1</i>, <i>MAL</i>, and <i>PAX1</i>, is implicated in cervical carcinogenesis. <b>Objectives:</b> This pilot study aimed to evaluate the methylation levels of these genes in HPV-p...
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Polyclonal antibody-based dot-ELISA and immunocapture-RT-PCR for Cucumber green mottle mosaic virus detection
Wydane 2010-09-01“…On the basis of the prepared PAb against CGMMV, a dot-ELISA and an immunocapture-RT-PCR (IC-RT-PCR) were established for CGMMV detection. …”Cucumber green mottle mosaic virus (CGMMV) is one of important viruses from cucurbit crops. Using purified CGMMV particles as immunogens, the polyclonal antibody (PAb) was prepared in New Zealand rabbits. The ELISA titer of the PAb was 1∶512 000. The PAb could specifically react with the 17.5 ku coa...
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Development of a SYBR Green-Based RT-qPCR Assay for the Detection and Quantification of Lone Star Virus
Wydane 2024-03-01Dokumenty pełnotekstoweLone Star virus (LSV) is a newly characterized arbovirus with pathogenic potential. However, no detection methods are available to specifically identify and monitor LSV. Two SYBR green-based RT-qPCRs were developed and validated for the detection and quantification of LSV. Two primer sets were evalu...
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Evaluation of Real Time PCR Detection of the 47 kDa Gene for Reliable Diagnosis of Pediatric Scrub Typhus
Wydane 2024-01-01“…This study assessed real time PCR detection of the 47 kDa gene along with routine IgM ELISA for confirmatory diagnosis of scrub typhus. …”Diagnosis of scrub typhus is difficult, because of its non-specific clinical features and a lack of reliable serological assays. This study assessed real time PCR detection of the 47 kDa gene along with routine IgM ELISA for confirmatory diagnosis of scrub typhus. This observational study was conduc...
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