Rezultaty - PCR detection
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Development of a sensitive and reliable droplet digital PCR assay for the detection of ‘Candidatus Liberibacter asiaticus’
Wydane 2018-02-01“…To better improve the detection sensitivity, a droplet digital PCR (ddPCR) assay was developed for the rapid detection of ‘Candidatus Liberibacter asiaticus’ (Las), the putative causal agent of HLB. …”Citrus Huanglongbing (HLB, yellow shoot disease) is one of the most serious citrus diseases worldwide. To better improve the detection sensitivity, a droplet digital PCR (ddPCR) assay was developed for the rapid detection of ‘Candidatus Liberibacter asiaticus’ (Las), the putative causal agent of HLB...
Dokumenty pełnotekstowe
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Development and validation of mPCR-CEFA for detecting multiple deletion and non-deletion thalassemia genotypes
Wydane 2025-07-01“…Rapid and accurate genotyping is essential for molecular screening and prenatal genetic diagnosis to prevent the birth of individuals with severe forms of the disease.MethodsWe developed a multiplex PCR-capillary electrophoresis fragment analysis (mPCR-CEFA) method to detect 16 α-thalassemia and 24 β-thalassemia genotypes simultaneously. …”BackgroundThalassemia is a common hereditary blood disorder caused by genetic variants in globin genes, leading to abnormal hemoglobin production. Rapid and accurate genotyping is essential for molecular screening and prenatal genetic diagnosis to prevent the birth of individuals with severe forms o...
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Novel multiplex PCR approach for the detection of Erysipelothrix rhusiopathiae, Streptococcus suis, and Staphylococcus hyicus in swine
Wydane 2025-06-01“…To address this, a novel multiplex PCR-based detection assay was optimized and evaluated for the simultaneous detection of E. rhusiopathiae, S. suis, and S. hyicus targeting the, 23S rRNA, recN, and sodA genes, respectively. …”Erysipelothrix rhusiopathiae, Streptococcus suis, and Staphylococcus hyicus are neglected, emerging and zoonotic pathogens in pigs that share common clinical signs and lesions, particularly septicaemia and skin lesions, highlighting the need for differential diagnosis. Currently, no single diagnosti...
Dokumenty pełnotekstowe
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Molecular detection of clarithromycin-resistant Helicobacter pylori in stool samples using real-time PCR
Wydane 2025-08-01Dokumenty pełnotekstoweBackground: Helicobacter pylori is a spiral-shaped bacterium that thrives in the digestive system. This bacterial infection is highly prevalent, it affects more than 50% of the world's population. It is common among children in developing countries. Transmission can occur through close persona...
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Development of A Real-Time PCR Assay for Plasmodiophora brassicae and Its Detection in Soil Samples
Wydane 2013-10-01“…A SYBR Green I real-time PCR assay was developed to detect and quantify Plasmodiophora brassicae ribosomal DNA (rDNA) and internal transcribed spacer (ITS). …”A SYBR Green I real-time PCR assay was developed to detect and quantify Plasmodiophora brassicae ribosomal DNA (rDNA) and internal transcribed spacer (ITS). A pair of primers PBF1/PBR1 was designed based on the conservative region of rDNA-ITS of P. brassicae. The positive plasmid pB12 was obtained a...
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Airborne SARS-CoV-2 Detection by ddPCR in Adequately Ventilated Hospital Corridors
Wydane 2025-07-01“…SARS-CoV-2 levels were quantified using droplet digital PCR. SARS-CoV-2 was detected in 60% of the total air samples. …”Indoors, the infection risk of diseases transmitted through the airborne route is estimated from indoor carbon dioxide (CO<sub>2</sub>) levels. However, the approaches to assess this risk do not account for the airborne concentration of pathogens, among other limitations. In this study,...
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Integration of Portable PCR and a Lateral Flow Assay for the Rapid Detection of HPV Type 16
Wydane 2025-06-01“…Although polymerase chain reaction (PCR) is considered the gold standard for HPV detection, its reliance on gel electrophoresis can be costly and require specialized facilities.Objective: We developed a diagnostic approach integrating PCR with a lateral flow assay to detect HPV type 16 using gold nanoparticles as visible labels.Methods: Primers targeting the L1 gene of HPV 16 were labeled with 6FAM (forward) and biotin (reverse). …”Muhammad Yusuf,1,2 Fauzian Giansyah Rohmatulloh,2 Siti Soidah,2 Shabarni Gaffar,1 Kemala Isnainiasih Mantilidewi,3 Gatot Nyarumenteng Adhipurnawan Winarno,3 Yudi Mulyana Hidayat,3 Toto Subroto1,2 1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Sumedang...
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Real-time PCR assay for detection of Dickeya fangzhongdai causing bleeding canker of pear disease in China
Wydane 2020-04-01“…The real-time PCR assay detected 0.2 pg μL−1 DNA and 1×103 CFU mL−1 of D. fangzhongdai. …”Bleeding canker, caused by Dickeya fangzhongdai, is a devastating disease of pear in China. The bacterium causes cankers, branch die-back, and eventually kills pear trees. The typical sign of bleeding canker infection is a rusty-brown bacterial ooze that exudes down from cankers onto branches or tru...
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Development of the PCR Assay with Universal Primers for the Detection of Different <i>tcpA</i> Gene Variants
Wydane 2012-02-01Hasła przedmiotowe: Dokumenty pełnotekstoweDeveloped is the PCR assay for the detection of the structural genes of toxin co-regulated adhesion piluses - tcpA of different types. Determined are the universal primers, the usage of which provides for the detection of the stated above genes in V. cholerae of various serogroups. With the help of...
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Molecular detection of Dientamoeba fragilis in children in southern Xinjiang, China
Wydane 2025-09-01Hasła przedmiotowe: Dokumenty pełnotekstoweDientamoeba fragilis is a common intestinal parasite in human and animals worldwide. In this study, 609 fecal samples were collected from preschool children in 11 counties in Southern Xinjiang, China. All samples were screened for D. fragilis using PCR targeting SSU rRNA gene, revealing an infection...
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The Quantitative Detection of Urogenital Mycoplasmas in Men with Urolithiasis
Wydane 2025-07-01“…., <i>M. genitalium</i> and <i>M. hominis</i> in men with urolithiasis using quantitative real-time PCR (qPCR). The study group comprised 100 men with urolithiasis. …”Urease-positive urogenital mycoplasmas are considered to be responsible for the formation of urinary stones. They are usually a part of the normal flora in the human urogenital tract, causing asymptomatic infections. However, many symptomatic infections with these bacteria have been reported. <i&...
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Detection of Arachnomelia Syndrome in Simmental Cattle in Türkiye
Wydane 2025-07-01Dokumenty pełnotekstoweABSTRACT Hereditary diseases in cattle lead to economic losses across various breeds worldwide, with arachnomelia syndrome being one such disorder found in Simmental cattle. Arachnomelia results from a recessively inherited loss‐of‐function variant in the MOCS1 gene detected only in Simmentals. In T...
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Molecular genetic techniques in current biomedical research. Part II: PCR applications in diagnostics of human infectious diseases
Wydane 2021-03-01Hasła przedmiotowe: Dokumenty pełnotekstowePolymerase chain reaction (PCR)-based diagnostics is currently established as a gold standard for the detection of microorganisms. The features of PCR include rapid amplification of DNA and RNA as well as high sensitivity and specificity. In contrast to diagnostic microbiology, PCR diagnostics does...
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CONCEPTUAL APPROACHES TO THE RAPID DETECTION OF CAMPYLOBACTER SPP. IN MEAT OF SLAUGHTER ANIMALS
Wydane 2018-01-01“…The priority method of investigation is PCR. Ready-to-use PCR test system was developed for the detection of Campylobacter spp. on the basis of selected gene-specific primers to bacteria of Campylobacter genus. …”The modern approach to quality assurance of food products based on the ISO 9000 standards indicates the need for the implementation of quality management systems in processing plants. According to the analysis of scientific publication databases (Science Direct and Web of Science), it is established...
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Development of a Molecular Platform for GMO Detection in Food and Feed on the Basis of “Combinatory qPCR” Technology
Wydane 2012Dokumenty pełnotekstoweNone
Elektroniczne Rozdział
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Detecting the Helicobacter pylori 16S rRNA Gene in Dyspepsia Patients Using Real-Time PCR
Wydane 2019-05-01“…The coccoid form of H. pylori is difficult to detect either by culture or histopathology; however, it can be detected using molecular methods, such as real-time PCR. …”Background: early detection of H. pylori is essential to prevent the development of infections into gastric malignancies. The coccoid form of H. pylori is difficult to detect either by culture or histopathology; however, it can be detected using molecular methods, such as real-time PCR. The study wa...
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Efficacy of a novel, multiplex, real-time PCR kit for the detection of Helicobacter pylori and clarithromycin resistance
Wydane 2025-08-01“…Clarithromycin resistance was detected in 12.5% of 112 samples, which were H. pylori positive by qPCR. …”Background: Helicobacter pylori is a pathogen involved in the etiopathogenesis of gastroduodenal infections and some gastric malignancies. Accurate diagnosis and rapid detection of antibiotic resistance are crucial for effective treatment. Methods: We investigated the efficacy of a novel, multiplex,...
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REQUIREMENTS FOR EFFICIENT PCR CLAMPING BY LOCKED NUCLEIC ACID OLIGONUCLEOTIES FOR SIMPLE AND SENSITIVE DETECTION OF SOMATIC MUTATIONS
Wydane 2018-09-01“…PCR clamping/wild-type blocking PCR with non-extendable locked nucleic acid (LNA) oligonucleotides is used for sensitive detection of somatic mutations in tumors. …”PCR clamping/wild-type blocking PCR with non-extendable locked nucleic acid (LNA) oligonucleotides is used for sensitive detection of somatic mutations in tumors. Various versions of the technique use different DNA polymerases and LNA oligonucleotides with and without additional phosphorothioate m...
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Tomato mottle mosaic virus: Characterization, resistance gene effectiveness, and quintuplex RT-PCR detection system
Wydane 2022-01-01“…These results highlight the threat posed by ToMMV to tomato and pepper cultivation and offer an efficient detection system for the simultaneous detection of four tobamoviruses and tomato spotted wilt virus infecting tomato plants in the field.…”Tomato mottle mosaic virus (ToMMV), an economically important species of the genus Tobamovirus, causes significant loss in yield and quality of tomato fruits. Here, we identified the Shandong isolate of ToMMV (ToMMV-SD) collected from symptomatic tomato fruits in Weifang, Shandong Province of China....
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Real-Time PCR Detection of <i>Bacillus anthracis</i> by Lambda_Ba03 Prophage Genes
Wydane 2022-10-01“…The aim of the study was to develop a set of primers and fluorescent probes for the detection of two chromosomal targets of Bacillus anthracis using real-time PCR based on the lambda_Ba03 prophage genes.Materials and methods. …”The aim of the study was to develop a set of primers and fluorescent probes for the detection of two chromosomal targets of Bacillus anthracis using real-time PCR based on the lambda_Ba03 prophage genes.Materials and methods. BLAST analysis of B. anthracis chromosomal DNA identified two target genes...
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