Rezultaty - PCR detection
-
21
Antibiotic-Resistant Strains of Plague Agent and Development of Procedure for Their Detection by PCR Method
Wydane 2011-02-01Dokumenty pełnotekstoweThe data of antibiotic-resistant strains of Yersinia pestis isolated from clinical sources and animals are shown in the present study. Highlighted are the reasons of their resistance to different drugs. Represented are two PCRs developed for detection of antibiotic resistance genes, their efficiency...
Artykuł -
22
Multiplex Real Time PCR Test-System for the Detection of Anthrax Causative Agent
Wydane 2013-03-01Hasła przedmiotowe: Dokumenty pełnotekstoweDesigned is a multiplex PCR-RT test-system for the detection of anthrax causative agent using hybridization fluorescence detection – “ Bacillus anthracis multiplex 3 FRT”. This test-system contains enclosed reference sample for carrying out control procedure in the phase of DNA pur...
Artykuł -
23
USING REAL-TIME PCR FOR URGENT DETECTION AND PROPHYLAXIS OF TICK-BORNE INFECTIONS
Wydane 2016-11-01“…The aim of this work was to evaluate the usability of real-time PCR (RT-PCR) assay in early detection of tick-borne infections. …”The urgent detection of tick-borne pathogens in attached ticks or in human blood followed by the prophylaxis of infection with specific immunoglobulin and/or antibiotics is one of the most reliable measures to prevent human disease. The aim of this work was to evaluate the usability of real-time PCR...
Dokumenty pełnotekstowe
Artykuł -
24
Application of droplet digital PCR in detection of seed-transmitted pathogen Acidovorax citrulli
Wydane 2020-02-01Hasła przedmiotowe: Dokumenty pełnotekstoweBacterial fruit blotch caused by Acidovorax citrulli is a serious threat to cucurbit industry worldwide. The pathogen is seed-transmitted, so seed detection to prevent distribution of contaminated seed is crucial in disease management. In this study, we adapted a quantitative real-time PCR (qPCR) as...
Artykuł -
25
DETECTION OF RHIZOBIUM VITIS AND R. RADIOBACTER STRAINS BY PCR WITH THE PRIMERS TO DIFFERENT GENOME SEQUENCES
Wydane 2011-06-01Dokumenty pełnotekstowePathogenic Rhizobium vitis and R. radiobacter strains in symptomless plants and grapevine tumors were revealed by PCR technique. The comparative tests with five primer pairs to different siquences of bacterial genome were carried out. It was shown that in plants of Cabernet Sauvignon cultivar 18.5%...
Artykuł -
26
Development of a multiplex reverse transcription-PCR assay for simultaneous detection of garlic viruses
Wydane 2015-05-01Hasła przedmiotowe: Dokumenty pełnotekstoweA preliminary screening for garlic viruses in garlic plants in Hunan, China, using existing monoplex (simplex) reverse transcription-polymerase chain reaction (RT-PCR) procedures detected four viruses/virus groups. These viruses/virus groups were Onion yellow dwarf virus (OYDV), Leek yellow stripe v...
Artykuł -
27
Detection of Microbial Growth on Indoor Building Materials in Two Countries Using qPCR
Wydane 2025-07-01“…Hence, qPCR has great potential to become a standard method of detecting microbes in indoor environments.…”According to several reports, 10–50% of buildings in Europe and worldwide suffer from moisture problems, which can lead to microbial growth in building materials. Unrepaired moisture and microbial damage can lead to the degradation of building structures and reduce visual appeal, resulting in econom...
Dokumenty pełnotekstowe
Artykuł -
28
-
29
Real time-PCR, ELISA, as an identification methods for Detection respiratory syncytial virus in children
Wydane 2023-01-01“…Nasopharyngeal Swab was more sensitive than the whole blood specimens for the detection of HRSV ,which is found in 1% by RT-PCR, in 81.9% by IgM –anti HRSV antibodies and in79.5% by IgG-HRSV antibodies. …”The study have been done to detecting HRSV infections among hospitalized children under 10 years old in Salahaldin Governorate, in addition to compare different diagnostic methods for detection of this virus. Nasopharyngeal Swabs (NPS) and whole blood samples were taken from 400 hospitalized chi...
Dokumenty pełnotekstowe
Artykuł -
30
Detection of Staphylococcus Enterotoxin sea and seb in Milk Samples by Duplex Droplet Digital PCR
Wydane 2025-07-01Hasła przedmiotowe: “…Duplex ddPCR…”This study sought to develop a method to accurately and quantitatively measure the staphylococcal enterotoxin genes sea and seb in milk samples using duplex droplet digital polymerase chain reaction (ddPCR). Specific primers and probes were designed for sea and seb. By optimizing the concentrations...
Dokumenty pełnotekstowe
Artykuł -
31
Genetic Markers for Detecting the DNA of Pathogenic Borrelia
Wydane 2022-07-01Dokumenty pełnotekstoweThe aim of the study was to analyze the genetic markers of Lyme disease pathogens, which can be used to specifically indicate maximum number of their strains and isolates. Materials and methods. The nucleotide sequences of various genes of Borrelia garinii, B. afzelii, B. burgdorferi were downloaded...
Artykuł -
32
An ultrasensitive and specific CRISPR-Cas13a-mediated point-of-care assay for monkeypox detection and PCR-based clade detection
Wydane 2025-06-01“…Compared with quantitative PCR (qPCR), three samples tested as positive using our assay, which showed that the performance of this assay was superior to that of the qPCR assay. …”Abstract Background The rapid increase in the number of monkeypox cases poses a considerable threat to the international community, necessitating sensitive, fast, and available diagnostic methods. Therefore, the objective of this study was to develop a rapid, sensitive and simple method with high cl...
Dokumenty pełnotekstowe
Artykuł -
33
Irrigation systems as reservoirs of diverse and pathogenic Pseudomonas syringae strains endangering crop health
Wydane 2025-09-01Hasła przedmiotowe: Dokumenty pełnotekstowePseudomonas syringae (Psy) is a widely distributed bacterial species complex primarily recognized as a foliar pathogen but also inhabits diverse environments, including water habitats, where strains closely related to agricultural pathogens have been identified. The connection between Psy-caused epi...
Artykuł -
34
Detection of the Most Common Genetic Causes of Male Infertility by Quantitative Fluorescent (QF)-PCR Analysis
Wydane 2011Dokumenty pełnotekstoweNone
Elektroniczne Rozdział
-
35
Detection of Pseudomonas aeruginosa in Clinical Samples Using PCR Targeting ETA and gyrB Genes
Wydane 2018-12-01“…To evaluate the accuracy and specificity of Polymerase Chain Reaction (PCR) assay, ETA and gyrB genes were targeted to detect pathogenic strains of P. aeruginosa. …”Pseudomonas aeruginosa has variety of virulence factors that contribute to its pathogenicity. Therefore, rapid detection with high accuracy and specificity is very important in the control of this pathogenic bacterium. To evaluate the accuracy and specificity of Polymerase Chain Reaction (PCR) assay...
Dokumenty pełnotekstowe
Artykuł -
36
Development of cell-based assay for detecting replication-competent adeno-associated virus by qPCR
Wydane 2025-09-01“…Here, we have developed a sensitive, cell-based assay for detection of rcAAV in AAV8 preparations. After transducing HEK293 cells over three rounds with AAV8 in the presence of helper adenovirus 5, we performed qPCR to detect the presence of rcAAV using the rep2 gene as a marker. …”Recombinant, non-replicative adeno-associated virus (AAV) containing a therapeutic gene of interest (GOI) is widely used as a vector for gene therapy. One way to manufacture AAV is through triple-transfection of HEK293 cells, with plasmids containing the (1) GOI, (2) replication (rep2) and capsid (c...
Dokumenty pełnotekstowe
Artykuł -
37
Comparison between ELISA and PCR for Detection of Epstein - Barr virus in children with acute cervical lymphadenitis
Wydane 2024-11-01“…Comparison between ELISA and PCR for Detection of Epstein - Barr virus in children with acute cervical lymphadenitis was done. …”Introduction: Among viruses, Epstein Barr Virus is an important cause of acute cervical lymphadenitis. The clinical features of acute Epstein-Barr virus infection overlap those of a variety of other infectious and non-infectious diseases so reliable laboratory tests are important to not only confir...
Dokumenty pełnotekstowe
Artykuł -
38
Development of nested-PCR for detection of Cryphonectria parasitica based on the marker of sequence characterized amplified region
Wydane 2015-01-01Hasła przedmiotowe: Dokumenty pełnotekstoweChestnut blight, caused by Cryphonectria parasitica, is a destructive disease on chestnut trees as well as an important international disease in the world. At the end of the 19th century and the beginning of the 20th century, this pathogen dispersed rapidly and nearly killed all the American chestnu...
Artykuł -
39
Development of Real-Time Multiplex PCR Assay for the Detection and Differentiation of Burkholderia mallei and Burkholderia pseudomallei
Wydane 2016-12-01“…Objective of the study was to develop a real-time multiplex PCR assay for the detection and differentiation of B. mallei and B. pseudomallei, characterized by high sensitivity and specificity. …”Objective of the study was to develop a real-time multiplex PCR assay for the detection and differentiation of B. mallei and B. pseudomallei, characterized by high sensitivity and specificity. Materials and Methods. The primers and probes were designed to detect the species-specific sequence of the...
Dokumenty pełnotekstowe
Artykuł -
40
Development of IC-RT real-time PCR method for detection of Cowpea severe mosaic virus
Wydane 2010-09-01“…Based on the RT real-time PCR, IC-RT real-time PCR detection method for CPSMV was set up. …”A method of immuno-capture reverse transcript real-time (IC-RT real-time) PCR assay was developed for detection of Cow pea severe mosaic virus (CPSMV). By introducing standard CPSMV strains from ATCC and DSMZ as materials, RT real-time PCR method was constituted firstly, which showed good amplificat...
Dokumenty pełnotekstowe
Artykuł