Rezultaty - PCR detection
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201
Identification and Characterization of Putative Virulent Genes in Streptococcus equi ssp. zooepidemicus
Wydane 2013-02-01Hasła przedmiotowe: Dokumenty pełnotekstoweSuppression subtractive hybridization (SSH) was performed with virulent strain ATCC35246 and avirulent strain ST171 to identify novel genes associated with virulence in Streptococcus equi ssp. zooepidemicus (SEZ). There were fourteen genomic regions that only presented in virulent strain ATCC35246....
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202
Transmission of potato spindle tuber viroid between <i>Phytophthora infestans</i> and host plants
Wydane 2022-06-01Hasła przedmiotowe: Dokumenty pełnotekstowePotato spindle tuber viroid (PSTVd) is a naked, circular, single-stranded RNA (356–363 nucleotides in length) which lacks any protein-coding sequences. It is an economically important pathogen and is classified as a high-risk plant quarantine disease. Moreover, it is known that PSTVd is mechanically...
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203
A Strand-Specific Quantitative RT-PCR Method for Detecting vRNA, cRNA, and mRNA of H7N9 Avian Influenza Virus in a Mouse Model
Wydane 2025-07-01“…Among the AIV subtypes posing public health threats, H7N9 AIV is responsible for five epidemic waves of human infection in China. Here, a detection system based on a mouse model was established, which can simultaneously and quantitatively analyze the dynamic changes in the viral genomic RNA (vRNA), complementary RNA (cRNA), and messenger RNA (mRNA) of H7N9 AIV by using reverse transcription primers with tag sequences to reverse transcribe the three species of RNAs into corresponding cDNA templates, which are then absolutely quantified using the TaqMan quantitative PCR method. …”Avian influenza virus (AIV) remains a persistent threat to both the poultry industry and human health. Among the AIV subtypes posing public health threats, H7N9 AIV is responsible for five epidemic waves of human infection in China. Here, a detection system based on a mouse model was established, wh...
Dokumenty pełnotekstowe
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204
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Assessment of a multiplex arbovirus PCR Detection Test in an area endemic for Chikungunya, Zika, and Dengue viruses: An evaluation of kit performance characteristics in line with Clinical Laboratory Improvement Amendments (CLIA) Standards.
Wydane 2025-01-01“…<h4>Introduction</h4>Several multiplex and singleplex PCR systems exist for the detection of Dengue(DENV), Zika(ZIKV), and Chikungunya(CHIKV) viruses; however, their performance characteristics in East Africa remain unassessed. …”<h4>Introduction</h4>Several multiplex and singleplex PCR systems exist for the detection of Dengue(DENV), Zika(ZIKV), and Chikungunya(CHIKV) viruses; however, their performance characteristics in East Africa remain unassessed. In this study, we investigated the TaqMan® Arbovirus Triplex...
Dokumenty pełnotekstowe
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206
Loop-mediated isothermal amplification (LAMP) method for fast detection of Campylobacter spp in meat food products and environmental objects of a processing plant’s
Wydane 2022-07-01Dokumenty pełnotekstoweThere is constant necessity of developing the accurate and fast methods for detection of foodborne pathogens. Microorganisms of Campylobacter genus are one of the main causes of foodborne diseases worldwide. Fast identification of Campylobacter at all stages of the food life cycle is an efficient st...
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207
The Detection and Differentiation of Pigeon Adenovirus Types 1 and 2 via a High-Resolution Melting Curve Platform
Wydane 2025-06-01Hasła przedmiotowe: Dokumenty pełnotekstoweTwo main adenoviral diseases have been described in pigeons: pigeon adenovirus type 1 (PiAdV-1) and pigeon adenovirus type 2 (PiAdV-2), which belong to the genus <i>Aviadenovirus</i> under the family <i>Adenoviridae</i>. PiAdV-1 and PiAdV-2 are highly pathogenic to pigeons, l...
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208
Highly Effective xMAP Multiplex Assay for the Detection and Identification of Hemorrhagic Fever Agents, Including Ebola Virus
Wydane 2015-09-01“…These results indicate that the xMAP multiplexing for the detection and identification of tropical hemorrhagic fever agents, including Ebola virus, is not inferior to the conventional method such as real-time RT-PCR and can be applied for evaluation of viral load, and further on can easily be expanded for both the analysis of new viral agents and for the detection of critical mutations in viral genomes.…”Developed has been the oligonucleotide liquid biochip based on xMAP technology, designed for the laboratory detection of particularly dangerous viral pathogens such as Ebola and Marburg filoviruses, and Machupo , Junin, and Lassa arenaviruses. The suggested approach allows for the detection of up to...
Dokumenty pełnotekstowe
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209
Detection of genetic markers of pathogenicity factors at associative symbiosis of Klebsiella spp. and Staphylococcus aureus autostrains, isolated from infants
Wydane 2014-09-01Dokumenty pełnotekstoweThe article presents the results of detection of genetic determinants of pathogenicity in 120 Klebsiella strains (Klebsiella pneumoniae, Klebsiella oxytoca) and 48 Staphylococcus aureus strains isolated from the intestinal habitat of infants with intestinal dysbiosis. Registration of the investigate...
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210
Comparative diagnostic efficacy of microscopy, combined flotation and polymerase chain reaction to detect <i>Giardia</i> spp. in dogs and cats
Wydane 2023-04-01“…The purpose of the research is to perform a comparative diagnostic efficacy of microscopy, combined flotation method and polymerase chain reaction (PCR) used to detect Giardia spp. in dogs and cats.Materials and methods. …”The purpose of the research is to perform a comparative diagnostic efficacy of microscopy, combined flotation method and polymerase chain reaction (PCR) used to detect Giardia spp. in dogs and cats.Materials and methods. The efficacy was compared between three methods to detect Giardia spp. on 60 fe...
Dokumenty pełnotekstowe
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211
Clinical study of real-time polymerase chain reaction test kit for SARS-CoV-2 ribonucleic acids detection
Wydane 2022-07-01“…Objective: development and validation of a reverse transcription polymerase chain reaction (RT-PCR) test kit for SARS-CoV-2 ribonucleic acids (RNA) qualitative detection adapted for using with automated station for RNA extraction.Material and methods. …”Objective: development and validation of a reverse transcription polymerase chain reaction (RT-PCR) test kit for SARS-CoV-2 ribonucleic acids (RNA) qualitative detection adapted for using with automated station for RNA extraction.Material and methods. Assessment of clinical performance was carried o...
Dokumenty pełnotekstowe
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DETECTION OF CAUSATIVE AGENTS OF TICK-BORNE INFECTIONS BY POLYMERASE CHAIN REACTION ASSAY CARRIED OUT AS A PART OF CLINICAL STUDIES IN THE “REPUBLICAN CLINICAL INFECTIOUS DISEASES...
Wydane 2018-07-01Dokumenty pełnotekstoweThe article is devoted to the study of vector-borne pathogens circulating in the Republic of Buryatia. The authors have carried out identification of the genetic material of tick-borne encephalitis virus, Borrellia burgdorferi, Borrellia miуamotoi, Coxiella burnetii and Rickettsia sibirica in var...
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213
Discovery of Genetic Markers of Resistance to β-Lactams in Gramnegative Microorganisms by PCR Diagnosis
Wydane 2020-05-01“…Monocultures of gramnegative bacteria resistant to cefotaxime, cefepime, imipenem and meropenem were isolated from the specimens. The PCR method with detection of the results in the real time regimen (PCR test-system Litekh) provided detection of the beta-lactamase genes: bla/CTX- M-like (72/104, 69.2%), blaNDM (6/104, 5.8%), blaVIM (49/104, 47,1%) and blaOXA48_like (59/104, 56.7%). …”Fifteen specimens of the hemoculture and 89 specimens of the broncho-alveolar lavage were used in the study. Monocultures of gramnegative bacteria resistant to cefotaxime, cefepime, imipenem and meropenem were isolated from the specimens. The PCR method with detection of the results in the real time...
Dokumenty pełnotekstowe
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214
Development of a non-infectious control for viral hemorrhagic fever PCR assays.
Wydane 2024-04-01“…Transcribed RNA was used as template in a dilution series, extracted and amplified with RT-PCR and RT-qPCR to demonstrate successful recovery and determine limits of detection in a range of laboratory settings. …”Assay validation is an essential component of disease surveillance testing, but can be problematic in settings where access to positive control material is limited and a safety risk for handlers. Here we describe a single non-infectious synthetic control that can help develop and validate the PCR ba...
Dokumenty pełnotekstowe
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215
Development of the Multiplex Real-Time PCR for Marburg, Ebola, and Lassa Viruses Identification
Wydane 2011-06-01“…Presented are the data on the development and approbation of the method of Marburg, Ebola, and Lassa viruses identification based on real-time multiplex PCR with hybridization-fluorescent detection. This method is meant for the differential diagnostics of hemorrhagic fevers caused by these viruses. …”Presented are the data on the development and approbation of the method of Marburg, Ebola, and Lassa viruses identification based on real-time multiplex PCR with hybridization-fluorescent detection. This method is meant for the differential diagnostics of hemorrhagic fevers caused by these viruses....
Dokumenty pełnotekstowe
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216
Development of an updated loop-mediated isothermal amplification (LAMP) assay for the rapid detection of Ralstonia syzygii subsp. celebesensis (Rsc), the causative agent of banana...
Wydane 2025-01-01“…This study developed and assessed a loopmediated isothermal amplification (LAMP), which detected as few as 10 cells per reaction, significantly surpassing conventional PCR, which required a minimum of 10³ cells per reaction. …”Efficient and accurate detection of Ralstonia syzygii subsp. celebesensis (Rsc), the pathogen responsible for banana blood disease, is vital for controlling its detrimental effects on banana cultivation. This study developed and assessed a loopmediated isothermal amplification (LAMP), which detected...
Dokumenty pełnotekstowe
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217
An isothermal workflow for low‐cost and PCR‐free field‐based community metabarcoding
Wydane 2025-07-01“…RPA‐based sequencing libraries equally detected complete composition and structure of communities when compared to PCR‐based approaches. …”Abstract DNA metabarcoding has revolutionized our ability to monitor ecosystems. However, the method is still rarely used in developing countries where resources are limited and fieldwork is challenging. To overcome this limitation, we designed a comprehensive workflow allowing rapid community metab...
Dokumenty pełnotekstowe
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218
Allele-specific PCR with fluorescently labeled probes: criteria for selecting primers for genotyping
Wydane 2024-05-01“…Whole-genome sequencing and genotyping by sequencing can detect SNPs with high specificity and identify novel variants. …”Single-nucleotide polymorphisms (SNPs) can serve as reliable markers in genetic engineering, selection, screening examinations, and other fields of science, medicine, and manufacturing. Whole-genome sequencing and genotyping by sequencing can detect SNPs with high specificity and identify novel vari...
Dokumenty pełnotekstowe
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219
An Integrated PMA Pretreatment Instrument for Simultaneous Quantitative Detection of <i>Vibrio parahaemolyticus</i> and <i>Vibrio cholerae</i> in Aquatic Products
Wydane 2025-06-01“…Leveraging this system, a duplex qPCR assay was optimized for simultaneous quantitative detection of <i>V. parahaemolyticus</i> and <i>V. cholerae</i> in aquatic products and environmental samples. …”Traditional hazard identification techniques for <i>Vibrio parahaemolyticus</i> often neglect the distinction between viable and nonviable bacteria in aquatic products, leading to overestimated disease risks and uncertainties in risk assessments. To address this limitation, we developed...
Dokumenty pełnotekstowe
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Genotypes Diversity and Virulence Factor screening of Trichomonas vaginalis Isolated from Pregnant Women in Mosul (North of Iraq)
Wydane 2022-10-01Dokumenty pełnotekstoweTrichomonas vaginalis is a causative agent of trichomoniasis , one of the most common non-viral sexually transmitted disease (STD) over all the world, especially in immunocompromised women such as pregnant. Wet smear and Giemsa stain are the current methods used in hospital to diagnosis trichomonia...
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