Rezultaty - PCR detection
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181
Comparison of microbial culture, metagenomic next-generation sequencing and droplet digital polymerase chain reaction methods for pathogen detection in patients with neurosurgical...
Wydane 2025-07-01“…Notably, the administration of empiric antibiotics did not significantly influence the positive detection rates of either mNGS or ddPCR. When stratified by infection type, mNGS and ddPCR demonstrated notably higher positive detection rates in three specific categories of NCNSIs-ventriculitis, intracranial abscess, and implant-associated infections-compared to meningitis. …”BackgroundNeurosurgical central nervous system infections (NCNSIs) are one of the most common complications in neurosurgical patients, followed by neurosurgery itself, trauma, implants or infection need to be treated by surgery. However, the diagnosis of NCNSIs continues to pose a significant challe...
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182
Assessing the necessity of technical replicates in reverse transcription quantitative PCR
Wydane 2025-04-01“…This study systematically evaluates the necessity of technical replicates by analyzing 71,142 cycle threshold (Ct) values from 1,113 RT-qPCR runs across three instruments, two detection chemistries, and 30 operators. …”Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is widely used for nucleic acid quantification. The use of technical triplicates in RT-qPCR aims to minimize variability and improve reliability but increases reagent consumption, labor, and time. This study systematically evalua...
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183
Plasma ddPCR for etiological diagnosis of focal bacterial infections
Wydane 2025-07-01“…BackgroundPlasma droplet digital polymerase chain reaction (ddPCR) has been used for pathogen detection and has shown good diagnostic value, but no studies have yet demonstrated its application in focal infections. …”BackgroundPlasma droplet digital polymerase chain reaction (ddPCR) has been used for pathogen detection and has shown good diagnostic value, but no studies have yet demonstrated its application in focal infections. Herein a pilot study using plasma ddPCR to diagnose focal infection is discussed.Meth...
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184
Detection rate for ESR1 mutations is higher in circulating‐tumor‐cell‐derived genomic DNA than in paired plasma cell‐free DNA samples as revealed by ddPCR
Wydane 2025-07-01Dokumenty pełnotekstowePlasma cell‐free DNA (cfDNA) analysis to track estrogen receptor 1 (ESR1) mutations is highly beneficial for the identification of tumor molecular dynamics and the improvement of personalized treatments for patients with metastatic breast cancer (MBC). Plasma‐cfDNA is, up to now, the most frequent l...
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185
Results of PCR diagnostics after gingiva soft tissue augmentation
Wydane 2022-10-01Dokumenty pełnotekstoweAim. To demonstrate the results of polymerase chain reaction in real time.Materials and methods. The gum fragment was placed in 1 ml of RNA-leiter ("QIAGEN", Germany), incubated for a day at +4C, samples were stored at -70 °C.To obtain cDNA from the RNA matrix, a ready-made set of reagents...
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186
Evaluation of a test-and-cull strategy to control bovine paratuberculosis based on the detection of Mycobacterium avium ssp. paratuberculosis DNA using the high-throughput Johne's...
Wydane 2025-08-01“…Therefore, control programs focused on early removal of infectious animals from a herd, combined with management approaches to reduce exposure of susceptible young animals, have a high likelihood of reducing the disease burden. Quantitative PCR (qPCR) tests to detect MAP in feces enable rapid and sensitive detection of infectious animals. …”ABSTRACT: Bovine Johne's disease (BJD) is a chronic, debilitating enteritis caused by Mycobacterium avium ssp. paratuberculosis (MAP), with both animal welfare and economic consequences. The detection of MAP in feces is an important measure of transmission from infectious animals through enviro...
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187
Assessment of the New Set of Reagents AmpliSens <i>Brucella spp</i>.-FL with Hybridization-Fluorescent Detection
Wydane 2011-06-01“…At CRIE, Moscow and RRAPI Microbe designed is the set of reagents for detection of Brucella spp. DNA in biological materials and cultures of microorganisms by means of PCR with hybridization-fluorescent registration of the results. …”At CRIE, Moscow and RRAPI Microbe designed is the set of reagents for detection of Brucella spp. DNA in biological materials and cultures of microorganisms by means of PCR with hybridization-fluorescent registration of the results. This reagent set, AmpliSens ® Brrucella spp.-FL, is supplied in two...
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188
The comparative analysis of BCR-ABL/ABL detection by real-time quantitative PCR and automated GeneXpert Dx System in chronic myeloid leukemia patients with major and complete molecular response
Wydane 2014-07-01Dokumenty pełnotekstoweKey words: chronic myeloid leukemia, BCR-ABL expression, automated GeneXpert method
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189
Development of a Strain-Specific Detection and Quantification Method for <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> HN019 Using WGS-SNP Analysis and qPCR
Wydane 2025-07-01“…To address the critical need for strain-specific detection, we developed a quantitative PCR (qPCR) assay targeting a unique single-nucleotide polymorphism (SNP) within the <i>gal</i>K gene, identified through comparative whole-genome sequencing (WGS) analysis of 31 <i>B. animalis</i> subsp. …”Accurate quantification of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> HN019, a clinically validated probiotic strain conferring immune modulation, gastrointestinal health, and gut barrier integrity benefits, is essential for diverse applications. To address the crit...
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190
Construction of the PCR Test-System for the Detection of <i>Vibrio cholerae</i> O1 Toxigenic Strains, for Indication of Their Biovar and for Differentiation between Typical and Altered El Tor-Vibrio Strains
Wydane 2011-08-01“…Worked out is the multilocus PCR test-system which makes it possible to identify Vibrio cholerae O1 strains on the basis of rfb gene detection, to determine their biovar - either Classical or El Tor by testing cas3 or rtxC genes respectively, and at the same time to differentiate them into typical (which carry ctxBEltor gene) and genetically altered (which carry ctxBClass gene) variants. …”Worked out is the multilocus PCR test-system which makes it possible to identify Vibrio cholerae O1 strains on the basis of rfb gene detection, to determine their biovar - either Classical or El Tor by testing cas3 or rtxC genes respectively, and at the same time to differentiate them into typical (...
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191
Diagnostic Accuracy of AdvanSure<sup>TM</sup> and PowerChek<sup>TM</sup> Real-Time PCR Assays for the Detection of <i>Mycobacterium tuberculosis</i> and Nontuberculous Mycobacteria
Wydane 2025-07-01“…This study compares the diagnostic performance of two commercial real-time PCR kits, AdvanSure<sup>TM</sup> TB/NTM and Kogene PowerChek<sup>TM</sup> MTB/NTM, for detecting MTB, NTM, and negative (no growth, NG) clinical specimens. …”<b>Background:</b> Accurate differentiation between <i>Mycobacterium tuberculosis</i> (MTB) and nontuberculous mycobacteria (NTM) is essential for proper diagnosis and treatment. This study compares the diagnostic performance of two commercial real-time PCR kits, AdvanSure<...
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192
Analysis of human parvovirus B19 nucleic acid detection in blood products in China
Wydane 2025-07-01“…[Conclusion] No human parvovirus B19 has been detected in human albumin, human intravenous immunoglobulin, or human rabies immunoglobulin. …”[Objective] To analyze the nucleic acid load of human parvovirus B19 in major commercially available blood products in China, including human albumin, human intravenous immunoglobulin, human rabies immunoglobulin and various coagulation factor products, aiming to provide evidence for improving blood...
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193
Evaluation of genetically modified rice detection methods 2011/884/EU and 2008/289/EC proposed by the European Union
Wydane 2016-12-01“…In our tests, 24.3% (18/74) of the samples tested were positive with the SYBR Green real-time PCR assay using the Decision 2011/884/EU method, but were negative with the TaqMan real-time PCR assay using the Decision 2011/884/EU and Decision 2008/289/EC methods. …”Increases in the number of cases of identified genetically modified (GM) rice contamination can be traced back to the first Rapid Alert System for Food and Feed (RASFF) in 2006. In response to the lack of reliable detection methods, Decision 2011/884/EU proposed that new screening methods replace De...
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194
Assessment of histology's performance compared with PCR in the diagnosis of Helicobacter pylori infection
Wydane 2024-12-01“…Aim: Histology is the most widely used test to detect H. pylori. PCR is less used but allows the detection of both infection and antibiotics' resistance. …”Aim: Histology is the most widely used test to detect H. pylori. PCR is less used but allows the detection of both infection and antibiotics' resistance. Methods: We conducted a monocentric cross-sectional study, collecting 97 symptomatic patients to assess the diagnostic performance of histolo...
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195
Diagnostic study for Toxoplasmosis by Serological test and PCR technique in Kirkuk province
Wydane 2018-04-01“…However, many molecular techniques are now being extensively used through the world among these methods polymerase chain reaction PCR technique emerged as the most widely accepted method for directly detection infectious agents. …”Background: Severe Toxoplasma gondii infection in early pregnancy brings the risk of transferring the infection to the unborn baby with serious abnormality. The aim of identifying toxoplasmosis in early stage is to recognize mother-to-infant transmission for early cure to avoid undesirable complica...
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196
New Approach for Diagnostics of VZV Infection by Using Real-Time PCR
Wydane 2016-10-01“…So the goal of this study was checking out usefulness of diagnostics based on detection viral DNA in clinical samples by real-time PCR method. …”Clinical picture of diseases caused by VZV in immunocompromised patients is often differed from healthy people and has no visible skin damage. Diagnostics in such cases is difficult but it is important to find out real reason of health deterioration for assignment the treatment. That explains useful...
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197
Application of PCR for Identification and Interspecific Differentiation of Glanders and Melioidosis Etiological Agents
Wydane 2008-06-01“…Assessed were possibilities of identification and differentiation of glanders and melioidosis etiological agents by means of PCR method using different nucleotide sequences of the pathogenic Burkholderia. …”Assessed were possibilities of identification and differentiation of glanders and melioidosis etiological agents by means of PCR method using different nucleotide sequences of the pathogenic Burkholderia. Primers perspective for inclusion in the test-system assigned for detection of glangers and mel...
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198
PCR-ANALYSIS OF GENOME CHANGEABILITY AND FUSARIUM FUNGI IDENTIFICATION TECHNOLOGY DEVELOPMENT
Wydane 2007-06-01“…It has been developed the PCR-detection system of fusaria with the help of genus-, species- and strain-sequences of DNA nucleotides.…”Fusarium moniliforme Sheldon var. lactis intrastrains variability research was carried out. We observed the changes in electrophoretic spectrums of the strains cultures that obtained in result of contact with fusarious resistant and sensible maize genotypes, by different cultivation terms and series...
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199
Differential PCR diagnosis of bacterial vaginosis, aerobic vaginitis and vulvovaginal candidiasis
Wydane 2022-06-01“…The study enrolled 240 women which flled out an original questionnaire on demographic, social, behavioral, and reproductive features and underwent a vaginal examination. Real-time PCR of vaginal smears was used to detect bacterial vaginosis, aerobic vaginitis, vulvovaginal candidiasis, and sexually transmitted infections (Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma genitalium).Results. …”Aim. To evaluate the effectiveness of PCR tests to diagnose common pathological conditions associated with vaginal discharge (i.e. bacterial vaginosis, aerobic vaginitis, and vulvovaginal candidiasis) in patients with and without complaints.Materials and Methods. The study enrolled 240 women which f...
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200
Difficulties of Q Fever Diagnostic Verification at Negative PCR Testing Results
Wydane 2023-04-01“…Results: the specific antibodies to C. burnetii antigens at negative results of PCR detected in 10 cases. The article provides the description of three clinical cases for demonstration of difficulties of coxiellosis diagnosis with analysis of serological profiles, titers and avidity of antibodies. …”Aim of the work: to demonstrate the difficulty of verifying the diagnosis of Q fever with negative results of PCR (DNA of Coxiella burnetii) in the blood and to assess the occurrence of serological markers among patients selected for this study based on a combination of clinical and epidemiological...
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