Torthaí cuardaigh - PCR detection

To detect Brucella inflection in milk, a nested-PCR assay was developed by designing outward-directed and inward-directed primers for omp25, omp31 and 16S rRNA genes of Brucella abortus, and optimizing the extraction of Brucella genomic DNA. The results show that two rounds of amplification with the...

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Hypervirulent Klebsiella pneumoniae (HvKP) is a notorious zoonotic pathogen that poses a significant threat to public health, as it can cause severe infections with high morbidity and mortality among young and healthy individuals. Commonly, a positive string test is primarily used to identify HvKP s...

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Background This study comparatively evaluated the analytical and diagnostic performance of a multiplex digital polymerase-chain reaction (dPCR) assay and a quantitative real-time PCR (qPCR) for the simultaneous detection and quantification of periodontal pathobionts: Porphyromonas gingivalis, Aggreg...

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The Edwardsiella tarda bacterium can infect a wide variety of fish species and is a common pathogen in aquaculture. Rapid and accurate detection of the pathogen is the premise and basis for its disease prevention and control. In this study, an aptamer with high affinity and specificity was used to b...

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Bacterial leaf blight of rice caused by Xanthomonas oryzae pv. oryzae (Xoo) and bacterial leaf streak of rice caused by Xanthomonas oryzae pv. oryzicola (Xoc) are two important bacterial diseases of rice. Identification of Xoo and Xoc belonging to the same species is critical for quarantine and cont...

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Meloidogyne vitis is a new root-knot nematode parasitic on grape root in Yunnan Province, China. In order to establish a rapid, reliable and specific molecular detection method for M. vitis, the species-specific primers were designed with rDNA-ITS (ribosomal DNA internal transcribed spacer) gene fra...

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A PCR assay for rapid and sensitive detection of haemolysin of Streptococcus suis type 2 was established. The PCR primers based on the haemolysin of S. suis type 2 succeeded in amplifying a 1502 bp PCR product. The PCR product could be digested into two fragments of 869 bp and 633 bp by EcoR I. With...

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To establish a PCR-based (polymerase chain reaction) method for the detection of Mycoplasma suis (M. suis) which was named Eperythrozoon suis, a pair of primers were designed according to the published sequence data of the 16S ribosomal RNA gene of M. suis. PCR product was cloned and sequenced. Sequ...

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This work focuses on evaluating nucleic acid extraction methods and optimizing lysis buffer components to enhance nucleic acid yield and minimize the impact of potential inhibitors on target gene amplification to improve the detection of causative pathogens and facilitate their integration into diag...

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Polymerase chain reaction is the most common laboratory method used in diagnosis of infectious diseases. It still requires cold-chain transportation and storage of reaction components, adequate instrumentation, as well as a skilled specialist during the preparation of reaction mixes. This study was...

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For detecting the harmful algal bloom (HAB) species sensitively and rapidly, Gymnodinium sanguineum was taken as the object, and the rapid detection method- RFQ-PCR (real-time fluorescent quantitative polymerase chain reaction) technique was applied to the HAB species detection. Firstly, gene specif...

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In order to observe the histopathological changes of the layer oviduct cysts infected with Gallibacterium anatis, target genes were amplified by PCR from genome DNA extracted from lung, trachea, oviduct and ovary of nine birds infected naturally, using genus-specificity primes of Gallibacterium anat...

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Enzyme immunoassay and polymerase chain reaction have become the «gold standard» for the detection of biological pathogens. The method of amplified immunoassay – immuno-PCR allows to combine both methods into a single platform to preserve their advantages and to achieve high sensitivity of the analy...

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Background: Leishmaniasis is a parasitic diseases that are spread worldwide due to various species of Leishmania, which are infect mammales diversity as well as human. L. tropica,    L. major, and L. aethiopica which is common causes of cutaneous Leishmaniasis in Salah Adeen and Baghdad provences....

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Antibiotic-resistant Vibrio strains in commonly consumed vegetables have been linked to recent cholera outbreaks. This study investigated the presence of antibiotic resistance and virulence genes in Vibrio species pumpkins, water leaves, and amaranth greens sold at public markets in Ondo...

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In view of the increasing interest in the Methicillin-resistant Staphylococcus aureus (MRSA). The extracted DNA yield was observed using the phenol-chloroform method, it ranged from (1.6-1.8) and concentration ranged from 100 to 800 ng/µl. Five classical enterotoxin genes were investigated in 20 is...

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During deep technological processing, DNA of food product components (specifically, in canned foods) is subjected to strong degradation, which makes the PCR-based food components identification more difficult. In this work, a primer-probe system is proposed, which was selected for the multi-copy reg...

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