Search Results - "vitrification"

Collections of common potato maintained in the field genebanks suffer significant losses due to the impact of extreme environmental factors, diseases and pests. The solution of the problem of safe long-term preservation of common potato accessions is to create doublet in vitro and cryo-collections....

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Background: Frozen embryo transfer (FET) has been a routine assisted reproductive technology (ART) procedure since the first FET by slow freezing. It is estimated that more than 2 million FET cycles are performed worldwide each year. The stepwise removal of cytotoxic cryoprotectants and rehydration...

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Background: Cryopreservation of immature testicular tissue is a suitable method for spermatogonial stem cell (SSC) preservation in prepubertal boys, who are at risk of infertility due to cancer treatments. Viable spermatozoa can be obtained by transplantation or in vitro culture of cryopreserved tes...

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In this study of Nigella sativa seeds Cryopreservation by vitrification. was studied. This experiment with three replications with two factors, both soluble dewatering (Pvs2 and Pvs3) times and dewatering of seeds before freezing process during the 7 period (20, 40, 60, 80, 100, 120 and 140 minutes)...

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Introduction: Vitrifying blastocysts immediately after trophectoderm biopsy is recommended in clinical settings. However, in some cases, embryologists cannot perform vitrification immediately after biopsy because of other tasks, during which the blastocysts may begin to re-expand. Aims: This study a...

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Cotyledon and leaf explants of two spring rapeseed varieties were transformed with Agrobacterium tumefaciens harboring a genetic construct with the gfp marker gene. In order to reduce the proportion of hyperhydrated shoots, which appeared during regenerant formation, we optimized sucrose content in...

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The embryonic cerebral cells (ECC) of pregnant SD rats (17-19 days) were cryopreserved by vitrification. After cryopreservation for 3,10,20,30 and 60 days, the individual ECC samples were separately thawed in 38℃ water bath or by microwave treatment in 300 mL 10℃ water under microwave power of 800W,...

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In recent years, assisted reproductive technologies in animal production have included artificial insemination in need of high-quality cryopreserved bull sperm. High-quality freezing depends on the optimally selected parameters of the semen diluent. In this connection, the purpose of this review is...

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Vitrification is a common technique for cryopreserving oocytes or embryos. However, manual vitrification is tedious and labor-intensive, and can be subject to variations caused by human factors. To address these challenges, we developed an automated vitrification-thawing system (AVTS) based on a cry...

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Cryopreservation of protoplasts and protocorms of Dendrobium candidum by vitrification were studied, their survival rates were 48% and 88% respectively. The results showed that material, pretreatment and some other factors were very important for survival.

The dissolution reactions between high level liquid waste (HLLW) calcines and molten basic glass are key to the HLLW vitrification by two-step cold crucible induction melter (CCIM) technology. The phase evolution occurring in the melts of glass and calcines at different melting temperatures and peri...

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The aim of the present study was to identify the influence of extra- (EOV) and intraovarian vitrification (IOV) on mitochondrial activity (MA) and chromatin state in porcine oocytes during maturation in vitro. During EOV porcine oocytes were exposed in cryoprotective solutions (CPS): CPS-1 – 0.7 M d...

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The preservation of testicular tissue and male germ cells represents a cutting-edge technique for safeguarding fertility, especially when sperm collection is not possible, such as in prepubertal animals, those that die unexpectedly or that receive gonadotoxic therapies after cancer detection, and in...

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Cryopreservation is a powerful tool for genetic preservation, assisted reproduction, and the conservation of valuable species [...]

Dissipative effects are investigated by means of relaxation spectrometry in films of polyvinyl alcohol with various molecular weight and hydration degree. Peaks of mechanical loss reveal two relaxation transitions (α- and β-peaks) and one first order phase transition (δ-peak). It is found that decre...

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Background: Frozen-thawed embryo transfer (FET) during the endometrial receptivity window is important for implantation. Objective: This study aims to compare the clinical pregnancy rate in 2 methods of endometrial preparation in FET using the hormone replacement cycle (HRC) and the modified nat...

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The present research work focuses on the characterization and leachability evaluation of electric arc furnace dust (EAFD) and its vitreous outgrowths produced during vitrification with soda lime recycled glass (SLRG). EAFD is a hazardous industrial waste generated in the collection of particulate ma...

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Nuclear power generation has been increasing worldwide over the years, helping to avoid the emission of billions of tons of carbon dioxide (CO2) compared to coal power generation, making it a reliable method for providing green energy. However, as a byproduct of nuclear reactors, as well as fuel pro...

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To our knowledge, no single study has systemically compared cryopreservation efficiencies of bovine blastocysts derived from in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI) and somatic cell nuclear transfer (SCNT) by controlled freezing and vitrification. This experiment, there...

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Shoot tips excised from two resources of Rhodiola sachalinensis A. Bor were cryopreserved by encapsulation-vitrification, and effects of sucrose preculture duration, osmoprotection time during encapsulation and exposure time to PVS₂ on survival rate of cryopreserved shoot tips...

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