Search Results - "high-throughput sequencing"

In the 15 years since High Throughput Sequencing (HTS) was first used for the detection and identification of plant viruses, the technology has matured and is now being used in frontline plant biosecurity applications. Anticipating the challenges this new approach was starting to reveal, recommendat...

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ABSTRACT Background Intervertebral disk (IVD) degeneration is associated with lower back pain and aging; however, the mechanisms underlying age‐related changes and the changes in macrophage polarization in aging intervertebral disks require further elucidation. The aim of this study was to evaluate...

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Sterculia foetida L. (1753), a tree species in the family Malvaceae, is widely cultivated in tropical regions  for its applications in food, medicine, and biodiesel production. This study reports the first complete chloroplast genome sequence of S. foetida, which is 162,637 bp long with a GC content...

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Introduction. Currently in routine clinical practice, standard methods based on polymerase chain reaction (PCR) and immunohistochemical examination are used to determine microsatellite instability (MSI) and deficient mismatch repair system (dMMR). MSI identification using high-throughput sequencing...

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We report a case of disseminated herpes zoster diagnosed by high-throughput sequencing. A 74-year-old female presented with a one-week history of abdominal pain and distension. Two months ago, she was diagnosed with ANCA-associated vasculitis and received immunosuppressive therapy. One week ago, she...

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This study was conducted to investigate the phylogenetic diversity of archaea in the rumen of adult and elderly yaks. Six domesticated female yaks, 3 adult yaks ((5.3±0.6) years old), and 3 elderly yaks ((10.7±0.6) years old), were used for the rumen contents collection. Illumina MiSeq high-throughp...

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The effect of nitrogen (N) fertilizer on the development of maize kernels has yet to be fully explored. MicroRNA-mRNA analyses could help advance our understanding of how kernels respond to N. This study analyzed the morphological, physiological, and transcriptomic changes in maize kernels under dif...

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Abstract Neolamarckia cadamba (Roxb.) Bosser (Rubiaceae) is a widespread plant with medicine value and has been used for treating various diseases, such as coughs, fevers, anemia, blood disorders and stomach pains. It had been reported that plant miRNAs might enter mammalian intestines and exert a s...

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The adaptive immune system can protect the body from various pathogens by producing a variety of antibodies. The antibody repertoire is so vast that the traditional low-throughput sequencing method cannot completely sequence it. In this study, the library of porcine B cell receptor (BCR) heavy chain...

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Cambarellus patzcuarensis is a popular aquarium freshwater crayfish in China. In this study, the mitochondrial genome of C. patzcuarensis was sequenced using Illumina HiSeq. The coding mitogenome of C. patzcuarensis is 15,000 bp in length, consisting 13 protein-coding genes (PCGs), 22 transfer RNAs,...

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Follicular atresia is a natural process of follicular degeneration in mammal ovaries, significantly impacting female reproductive potential. However, the underlying regulatory mechanisms remain underexplored, particularly those involving non-coding RNAs like PIWI-interacting RNAs (piRNAs). In this s...

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The microbial community is one of the key indicators for evaluating the health of alpine marsh wetlands, and understanding the composition and health of alpine wetland communities provides a scientific rationale for conservation and restoration efforts. Taking the alpine marsh wetlands in Qilian Mou...

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In order to investigate the structural composition and dynamics of culturable microbial community in chestnut fruits, the present study was conducted to collect live microorganisms from two chestnut samples (coded as H and D) stored at 25, 4 or −18 ℃ for different periods (0, 14 and 30 days). The li...

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Long-term monoculture leads to continuous cropping (CC) problems, which complicate agricultural production, both locally and abroad. This study contrasted the different bacterial community compositions, physicochemical properties and enzyme activities of strawberry soil subjected to CC, CC rhizosphe...

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Abstract Houttuynia cordata Thunb (Family: Saururaceae) is well known as a traditional medicine plant and widely spread in China, Japan and India. It had been reported to have potential functions such as anti-bacterial, anti-cancer, and anti-inflammatory in human. Recently, it has been reported that...

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Evidence from an increasing number of studies indicates that plant endophytic microorganisms play a significant role during biotic and abiotic stress resistance. To date, however, only a handful of studies on endophytes in response to the presence of phytoplasmas have been conducted. The production...

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Carotid paragangliomas (CPGLs) are rare neuroendocrine tumors that arise from paraganglionic tissue of the carotid body localizing at the bifurcation of carotid artery. These tumors are slowly growing, but occasionally they become aggressive and metastatic. Surgical treatment remains high-risk and e...

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Soil microbiome makes a significant contribution to the implementation of ecosystem services, which are necessary for the sustainable functioning of ecosystems. Soils of central Yakutia develop under dynamic physical and chemical conditions (long-term freezing/thawing processes, redistribution of nu...

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The common questions in the design of the highthroughput sequencing experiments using RNA-Seq or Ribo-Seq methods are reviewed. The ENCODE guidelines (2011) as well as the recently published advances in the design of the studies of mammalian, animal and plant transcriptomes are also summarized in th...

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ABSTRACT Background and Objectives We aimed to conduct a systematic review and meta‐analysis of high‐throughput sequencing studies to assess changes in microbiome alpha, beta diversity, and composition differences in patients with human papillomavirus (HPV) infection and cervical cancer. Methods The...

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