תוצאות חיפוש - "PCR detection"

A PCR assay for rapid and sensitive detection of haemolysin of Streptococcus suis type 2 was established. The PCR primers based on the haemolysin of S. suis type 2 succeeded in amplifying a 1502 bp PCR product. The PCR product could be digested into two fragments of 869 bp and 633 bp by EcoR I. With...

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Tomato mottle mosaic virus (ToMMV), an economically important species of the genus Tobamovirus, causes significant loss in yield and quality of tomato fruits. Here, we identified the Shandong isolate of ToMMV (ToMMV-SD) collected from symptomatic tomato fruits in Weifang, Shandong Province of China....

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The aim of the study was to develop a set of primers and fluorescent probes for the detection of two chromosomal targets of Bacillus anthracis using real-time PCR based on the lambda_Ba03 prophage genes.Materials and methods. BLAST analysis of B. anthracis chromosomal DNA identified two target genes...

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Tomato brown rugose fruit virus (ToBRFV) is a novel tobamovirus firstly reported in 2015 and poses a severe threat to the tomato industry. So far, it has spread to 10 countries in America, Asia, and Europe. In 2019, ToBRFV was identified in Shandong Province (ToBRFV-SD), China. In this study, it was...

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Diagnosis of scrub typhus is difficult, because of its non-specific clinical features and a lack of reliable serological assays. This study assessed real time PCR detection of the 47 kDa gene along with routine IgM ELISA for confirmatory diagnosis of scrub typhus. This observational study was conduc...

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Developed is the PCR assay for the detection of the structural genes of toxin co-regulated adhesion piluses - tcpA of different types. Determined are the universal primers, the usage of which provides for the detection of the stated above genes in V. cholerae of various serogroups. With the help of...

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ABSTRACT Candida auris, a yeast that is highly resistant to antifungal treatments, represents a significant healthcare concern due to its rapid spread through surface contamination and patient colonization. A recent outbreak at the Miguel Servet University Hospital in Zaragoza, Spain, highlighted th...

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Influenza virus infection remains the leading cause of morbidity and mortality in the autumn-winter period. Along with insights into circulating virus subtypes and seasonal trends, reliable data on the prevalence of influenza among patients with acute respiratory illnesses is essential for guiding t...

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Suppression subtractive hybridization (SSH) was performed with virulent strain ATCC35246 and avirulent strain ST171 to identify novel genes associated with virulence in Streptococcus equi ssp. zooepidemicus (SEZ). There were fourteen genomic regions that only presented in virulent strain ATCC35246....

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Potato spindle tuber viroid (PSTVd) is a naked, circular, single-stranded RNA (356–363 nucleotides in length) which lacks any protein-coding sequences. It is an economically important pathogen and is classified as a high-risk plant quarantine disease. Moreover, it is known that PSTVd is mechanically...

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Stem or black rust of wheat, caused by the fungus Puccinia graminis Pers. f. sp. tritici Eriks. & E. Henn. (Pgt), has historically caused severe losses to wheat (Triticum aestivum L.) production worldwide. In the Fujian and Guangdong provinces of China, six moderate-to-severe epidemics of wh...

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<h4>Introduction</h4>Several multiplex and singleplex PCR systems exist for the detection of Dengue(DENV), Zika(ZIKV), and Chikungunya(CHIKV) viruses; however, their performance characteristics in East Africa remain unassessed. In this study, we investigated the TaqMan® Arbovirus Triplex...

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<i>Aerococcus urinaeequi</i> is an opportunistic pathogen that has been isolated from humans, pigs, and chickens, but with no reports in geese until now. This research aimed to isolate and identify <i>A. urinaeequi</i> from four geese, and establish a specific PCR detection m...

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Objective To evaluate the diagnostic value of reverse transcription-quantitative polymerase chain reaction (RT-qPCR) detection of skin pus specimens in HIV-negative patients with talaromycosis marneffei (TSM). Methods Twenty-seven patients who had suspected TSM (22 confirmed TSM and 5 non-TSM, all H...

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A method of immuno-capture reverse transcript real-time (IC-RT real-time) PCR assay was developed for detection of Cow pea severe mosaic virus (CPSMV). By introducing standard CPSMV strains from ATCC and DSMZ as materials, RT real-time PCR method was constituted firstly, which showed good amplificat...

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A polymerase chain reaction (PCR) method was developed to detect Melissococcus pluton and an epidemiological investigation of European foulbrood (EFB) was done in the main bee (Apis cerana cerana) breeding area of Chun’an County, Zhejiang Province. Results indicated that the newly developed method w...

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BIO ORGANS (BIO) gene is located on the short arm of Chromosome Ⅳ in Lotus japonicus. The bio mutant is obtained from a large scale EMS mutagenesis screen. Gene sequencing have proved that a stop coden appeared earlier in the transcripts of BIO by a retrotransposon insertion, which led to a truncate...

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A novel multiple target plasmid (MTP) molecule that satisfies the identification of specific transgenic soybean transformants in China was constructed. Fourteen transformants approved for import into China and four ones unauthorized but have important application prospects, were chosen as the target...

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Environmental DNA (eDNA) has proven an invaluable tool in detecting elusive, endangered, or otherwise hard-to-identify species in aquatic ecosystems. The Eastern hellbender salamander (Cryptobranchus alleganiensis) is a fully aquatic amphibian with an increasingly threatened population due to its re...

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